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Professor David Middleton

Professor

  1. 2020
  2. Published

    Raman Spectroscopy with 2D Perturbation Correlation Moving Windows for the Characterization of Heparin–Amyloid Interactions

    Townsend, D., Middleton, D. & Ashton, L., 31/10/2020, In: Analytical Chemistry. 92, 20, p. 13822–13828 7 p.

    Research output: Contribution to journalJournal articlepeer-review

  3. Published

    Sensitive morphological characterization of oriented high‐density lipoprotein nanoparticles using 31P NMR spectroscopy

    Lau, S. & Middleton, D., 5/10/2020, In: Angewandte Chemie International Edition. 59, 41, p. 18126-18130 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  4. E-pub ahead of print

    Aggregation Kinetics and Filament Structure of a Tau Fragment Are Influenced by the Sulfation Pattern of the Cofactor Heparin

    Townsend, D., Fullwood, N., Yates, E. & Middleton, D., 21/09/2020, In: Biochemistry.

    Research output: Contribution to journalJournal articlepeer-review

  5. E-pub ahead of print

    Circular dichroism spectroscopy identifies the β-adrenoceptor agonist salbutamol as a direct inhibitor of tau filament formation in vitro.

    Middleton, D., Townsend, D., Hughes, E., Fullwood, N., Siligardi, G., Hussain, R. & Mala, B., 10/06/2020, In: ACS Chemical Neuroscience.

    Research output: Contribution to journalJournal articlepeer-review

  6. 2019
  7. Published

    Drug orientations within statin-loaded lipoprotein nanoparticles by 19F solid-state NMR

    Lau, S., Griffin, J., Hughes, E. & Middleton, D., 14/11/2019, In: Chemical Communications. 55, 88, p. 13287-13290 4 p.

    Research output: Contribution to journalJournal articlepeer-review

  8. 2018
  9. Published

    Orientation of a Diagnostic Ligand Bound to Macroscopically Aligned Amyloid-β Fibrils Determined by Solid-State NMR

    Townsend, D. J., Hughes, E., Stewart, K., Griffin, J. M., Radford, S. & Middleton, D. A., 15/11/2018, In: The Journal of Physical Chemistry Letters. 9, 22, p. 6611–6615 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  10. Published

    Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin

    Townsend, D., Hughes, E., Akien, G., Stewart, K. L., Radford, S. E., Rochester, D. & Middleton, D. A., 17/08/2018, In: Journal of Biological Chemistry. 293, 33, p. 12877-12893 17 p.

    Research output: Contribution to journalJournal articlepeer-review

  11. Published

    Average orientation of a fluoroaromatic molecule in lipid bilayers from DFT-informed NMR measurements of 1H-19F dipolar couplings

    Hughes, E., Griffin, J. M., Coogan, M. P. & Middleton, D. A., 21/07/2018, In: Physical Chemistry Chemical Physics. 20, 27, p. 18207-18215 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  12. 2017
  13. Published

    Molecular Origins of the Compatibility Between Glycosaminoglycans and Aβ40 Amyloid Fibrils

    Stewart, K. L., Hughes, E., Yates, E. A., Middleton, D. A. & Radford, S. E., 4/08/2017, In: Journal of Molecular Biology. 429, 16, p. 2449-2462 14 p.

    Research output: Contribution to journalJournal articlepeer-review

  14. Published

    Heparin and methionine oxidation promote the formation of apolipoprotein A-I amyloid comprising α-helical and β-sheet structures.

    Townsend, D. J., Hughes, E., Hussain, R., Siligardi, G., Baldock, S. J., Madine, J. & Middleton, D. A., 21/03/2017, In: Biochemistry. 56, 11, p. 1632-1644 13 p.

    Research output: Contribution to journalJournal articlepeer-review

  15. 2016
  16. Published

    Atomic details of the interactions of Glycosaminoglycans with amyloid-β fibrils

    Stewart, K., Hughes, E., Yates, E., Akien, G. R., Huang, T-Y., Lima, M., Rudd, T., Guerrini, M., Hung, S-C., Radford, S. & Middleton, D. A., 13/07/2016, In: Journal of the American Chemical Society. 138, 27, p. 8328-8331 4 p.

    Research output: Contribution to journalJournal articlepeer-review

  17. Published

    Novel stereoselective bufadienolides reveal new insights into the requirements for Na+, K+-ATPase inhibition by cardiotonic steroids

    Tang, H-J., Ruan, L-J., Tian, H-Y., Liang, G-P., Ye, W-C., Hughes, E., Esmann, M., Fedosova, N. U., Chung, T-Y., Tzen, J. T. C., Jiang, R-W. & Middleton, D. A., 5/07/2016, In: Scientific Reports. 2016, 6, 10 p., 29155.

    Research output: Contribution to journalJournal articlepeer-review

  18. 2015
  19. Published

    Long-range effects of Na+ binding in Na,K-ATPase reported by ATP

    Middleton, D. A., Fedosova, N. U. & Esmann, M., 1/12/2015, In: Biochemistry. 54, 47, p. 7041-7047 7 p.

    Research output: Contribution to journalJournal articlepeer-review

  20. Published

    Comparisons with amyloid-β reveal an aspartate residue that stabilizes fibrils of the aortic amyloid peptide medin

    Davies, H., Madine, J. & Middleton, D., 20/03/2015, In: Journal of Biological Chemistry. 290, p. 7791-7803 13 p.

    Research output: Contribution to journalJournal articlepeer-review

  21. Published

    Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement.

    Whittaker, C., Patching, S., Esmann, M. & Middleton, D., 7/03/2015, In: Organic and Biomolecular Chemistry . 13, 9, p. 2664-2668 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  22. 2014
  23. Published

    Comparison of the structure and function of phospholamban and the Arginine-14 deficient mutant associated with dilated cardiomyopathy

    Hughes, E. & Middleton, D. A., 16/09/2014, In: PLoS ONE. 9, 9, 10 p., 106746.

    Research output: Contribution to journalJournal articlepeer-review

  24. Published

    Synthesis of bufalin derivatives with inhibitory activity against prostate cancer cells

    Yuan, X-F., Tian, H-Y., Li, J., Jin, L., Jiang, S-T., Liu, K. W-K., Luo, C., Middleton, D. A., Esmann, M., Ye, W-C. & Jiang, R-W., 3/06/2014, In: Natural Product Research. 28, 11, p. 843-847 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  25. Published

    Expression and purification of the aortic amyloid polypeptide medin

    Davies, H. A., Wilkinson, M. C., Gibson, R. P. & Middleton, D. A., 06/2014, In: Protein Expression and Purification. 98, p. 32-37 6 p.

    Research output: Contribution to journalJournal articlepeer-review

  26. Published

    Structures, chemotaxonomic significance, cytotoxic and Na+, K+-ATPase inhibitory activities of new cardenolides from Asclepias curassavica

    Zhang, R-R., Tian, H-Y., Tan, Y-F., Chung, T-Y., Sun, X-H., Xia, X., Ye, W-C., Middleton, D. A., Fedosova, N., Esmann, M., Tzen, J. T. C. & Jiang, R-W., 2014, In: Organic and Biomolecular Chemistry . 12, 44, p. 8919-8929 11 p.

    Research output: Contribution to journalJournal articlepeer-review

  27. 2013
  28. Published

    Heparin promotes the rapid fibrillization of a peptide with low intrinsic amyloidogenicity

    Madine, J., Davies, H. A., Hughes, E. & Middleton, D. A., 17/12/2013, In: Biochemistry. 52, 50, p. 8984-8992 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  29. Published

    Insights into the Molecular Architecture of a Peptide Nanotube Using FTIR and Solid-State NMR Spectroscopic Measurements on an Aligned Sample

    Middleton, D. A., Madine, J., Castelletto, V. & Hamley, I. W., 27/09/2013, In: Angewandte Chemie International Edition. 52, 40, p. 10537-10540

    Research output: Contribution to journalJournal articlepeer-review

  30. Published

    Probing the contacts of a low-affinity substrate with a membrane-embedded transport protein using 1H-13C cross-polarisation magic-angle spinning solid-state NMR

    Patching, S. G., Henderson, P. J. F., Sharples, D. J. & Middleton, D. A., 03/2013, In: Molecular Membrane Biology. 30, 2, p. 129-37 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  31. 2012
  32. Published

    Site-specific identification of an aβ fibril-heparin interaction site by using solid-state NMR spectroscopy

    Madine, J., Pandya, M. J., Hicks, M. R., Rodger, A., Yates, E. A., Radford, S. E. & Middleton, D. A., 21/12/2012, In: Angewandte Chemie International Edition. 51, 52, p. 13140-3 4 p.

    Research output: Contribution to journalJournal articlepeer-review

  33. Published

    Fibrils and nanotubes assembled from a modified amyloid-β peptide fragment differ in the packing of the same β-sheet building blocks

    Madine, J., Davies, H. A., Shaw, C., Hamley, I. W. & Middleton, D. A., 21/03/2012, In: Chemical Communications. 48, 24, p. 2976-8 3 p.

    Research output: Contribution to journalJournal articlepeer-review

  34. Published

    Amyloid-derived peptide forms self-assembled monolayers on gold nanoparticle with a curvature-dependent β-sheet structure

    Shaw, C. P., Middleton, D. A., Volk, M. & Lévy, R., 28/02/2012, In: ACS Nano. 6, 2, p. 1416-26 11 p.

    Research output: Contribution to journalJournal articlepeer-review

  35. Published

    Solid-state NMR reveals differences in the packing arrangements of peptide aggregates derived from the aortic amyloid polypeptide medin

    Davies, H. A., Madine, J. & Middleton, D. A., 01/2012, In: June 2002. ISSN 1460-4582. 18, 1, p. 65-72 8 p.

    Research output: Contribution to journalJournal articlepeer-review

  36. 2011
  37. Published

    The conformation of ATP within the Na,K-ATPase nucleotide site: a statistically constrained analysis of REDOR solid-state NMR data

    Middleton, D. A., Hughes, E. & Esmann, M., 25/07/2011, In: Angewandte Chemie International Edition. 50, 31, p. 7041-4 4 p.

    Research output: Contribution to journalJournal articlepeer-review

  38. Published

    A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain

    Hughes, E., Whittaker, C. A. P., Barsukov, I. L., Esmann, M. & Middleton, D. A., 04/2011, In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1808, 4, p. 1021-31 11 p.

    Research output: Contribution to journalJournal articlepeer-review

  39. Published

    Solid-state NMR detection of 14N-13C dipolar couplings between amino acid side groups provides constraints on amyloid fibril architecture

    Middleton, D. A., 02/2011, In: June 2002. ISSN 1460-4582. 49, 2, p. 65-9 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  40. 2010
  41. Published

    Measurement of multiple torsional angles from one-dimensional solid-state NMR spectra: application to the conformational analysis of a ligand in its biological receptor site

    Edwards, R., Madine, J., Fielding, L. & Middleton, D. A., 14/11/2010, In: June 2002. ISSN 1460-4582. 12, 42, p. 13999-4008 10 p.

    Research output: Contribution to journalJournal articlepeer-review

  42. Published

    Heparin-derived oligosaccharides interact with the phospholamban cytoplasmic domain and stimulate SERCA function

    Hughes, E., Edwards, R. & Middleton, D. A., 22/10/2010, In: Biochemical and Biophysical Research Communications. 401, 3, p. 370-5 6 p.

    Research output: Contribution to journalJournal articlepeer-review

  43. Published

    Comparison of aggregation enhancement and inhibition as strategies for reducing the cytotoxicity of the aortic amyloid polypeptide medin

    Madine, J. & Middleton, D. A., 08/2010, In: June 2002. ISSN 1460-4582. 39, 9, p. 1281-8 8 p.

    Research output: Contribution to journalJournal articlepeer-review

  44. 2009
  45. Published

    Evaluation of beta-alanine- and GABA-substituted peptides as inhibitors of disease-linked protein aggregation

    Madine, J., Wang, X., Brown, D. R. & Middleton, D. A., 17/08/2009, In: June 2002. ISSN 1460-4582. 10, 12, p. 1982-7 6 p.

    Research output: Contribution to journalJournal articlepeer-review

  46. Published

    Inhibitors of protein aggregation and toxicity

    Amijee, H., Madine, J., Middleton, D. A. & Doig, A. J., 08/2009, In: Biochemical Society Transactions. 37, Pt 4, p. 692-6 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  47. Published

    Structural analysis of uniformly (13)C-labelled solids from selective angle measurements at rotational resonance

    Patching, S. G., Edwards, R. & Middleton, D. A., 08/2009, In: June 2002. ISSN 1460-4582. 199, 2, p. 242-6 5 p.

    Research output: Contribution to journalJournal articlepeer-review

  48. Published

    Solid-state NMR studies of adenosine 5'-triphosphate freeze-trapped in the nucleotide site of Na,K-ATPase

    Middleton, D. A., Hughes, E., Fedosova, N. U. & Esmann, M., 20/07/2009, In: June 2002. ISSN 1460-4582. 10, 11, p. 1789-92 4 p.

    Research output: Contribution to journalJournal articlepeer-review

  49. Published

    Exploiting a (13)C-labelled heparin analogue for in situ solid-state NMR investigations of peptide-glycan interactions within amyloid fibrils

    Madine, J., Clayton, J. C., Yates, E. A. & Middleton, D. A., 7/06/2009, In: Organic and Biomolecular Chemistry . 7, 11, p. 2414-20 7 p.

    Research output: Contribution to journalJournal articlepeer-review

  50. Published

    Cross-beta spine architecture of fibrils formed by the amyloidogenic segment NFGSVQFV of medin from solid-state NMR and X-ray fiber diffraction measurements

    Madine, J., Copland, A., Serpell, L. C. & Middleton, D. A., 14/04/2009, In: Biochemistry. 48, 14, p. 3089-99 11 p.

    Research output: Contribution to journalJournal articlepeer-review

  51. Published

    Cytoplasmic residues of phospholamban interact with membrane surfaces in the presence of SERCA: a new role for phospholipids in the regulation of cardiac calcium cycling?

    Hughes, E., Clayton, J. C. & Middleton, D. A., 02/2009, In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1788, 2, p. 559-66 8 p.

    Research output: Contribution to journalJournal articlepeer-review

  52. 2008
  53. Published

    Structural insights into the polymorphism of amyloid-like fibrils formed by region 20-29 of amylin revealed by solid-state NMR and X-ray fiber diffraction

    Madine, J., Jack, E., Stockley, P. G., Radford, S. E., Serpell, L. C. & Middleton, D. A., 12/11/2008, In: Journal of the American Chemical Society. 130, 45, p. 14990-5001 12 p.

    Research output: Contribution to journalJournal articlepeer-review

  54. Published

    Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR

    Patching, S. G., Psakis, G., Baldwin, S. A., Baldwin, J., Henderson, P. J. F. & Middleton, D. A., 09/2008, In: Molecular Membrane Biology. 25, 6-7, p. 474-84 11 p.

    Research output: Contribution to journalJournal articlepeer-review

  55. Published

    The effects of alpha-synuclein on phospholipid vesicle integrity: a study using 31P NMR and electron microscopy

    Madine, J., Hughes, E., Doig, A. J. & Middleton, D. A., 09/2008, In: Molecular Membrane Biology. 25, 6-7, p. 518-27 10 p.

    Research output: Contribution to journalJournal articlepeer-review

  56. Published

    Design of an N-methylated peptide inhibitor of alpha-synuclein aggregation guided by solid-state NMR

    Madine, J., Doig, A. J. & Middleton, D. A., 25/06/2008, In: Journal of the American Chemical Society. 130, 25, p. 7873-81 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  57. Published

    Solid-state NMR spectroscopy detects interactions between tryptophan residues of the E. coli sugar transporter GalP and the alpha-anomer of the D-glucose substrate

    Patching, S. G., Henderson, P. J. F., Herbert, R. B. & Middleton, D. A., 30/01/2008, In: Journal of the American Chemical Society. 130, 4, p. 1236-44 9 p.

    Research output: Contribution to journalJournal articlepeer-review

  58. Published

    The dynamics and orientation of a lipophilic drug within model membranes determined by 13C solid-state NMR

    Boland, M. P. & Middleton, D. A., 7/01/2008, In: Physical Chemistry Chemical Physics. 10, 1, p. 178-85 8 p.

    Research output: Contribution to journalJournal articlepeer-review

  59. Published

    Membrane interactions of peptides representing the polybasic regions of three Rho GTPases are sensitive to the distribution of arginine and lysine residues

    Jack, E. R., Madine, J., Lian, L-Y. & Middleton, D. A., 01/2008, In: Molecular Membrane Biology. 25, 1, p. 14-22 9 p.

    Research output: Contribution to journalJournal articlepeer-review

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