Navigation signal simulator, which can be used as standard equipment for generating satellite navigation signals, can provide a real-like environment for the research and testing of navigation receivers. With the development of a variety of new navigation systems and signal standards, there is a higher demand for the compatibility and renewal speed of the navigation signal simulators; specifically, it should be multi-mode and reconfigurable in order to realize the flexible design of navigation systems. In this paper, a reconfigurable platform for navigation signal simulation is proposed and studied, aiming to simulate multi-mode and multi-frequency navigation signals. The performance of the proposed platform is validated from three perspectives: mathematic simulation software, output signals and receiver operation. The result shows that the platform can generate multi-mode and multi-frequency navigation signals through reconfiguration of the software and hardware on the same platform, hence supporting design of a number of navigation systems including Beidou, Global Positioning System (GPS), Russian Global Navigation Satellite System (GLONASS) and European Galileo system. The GNS8000 series navigation satellite signal simulator, designed based on such platform, have been successfully applied to the Beidou navigation satellite systems and widely used in the related fields of research and industries.