Assessing metal bioavailability in soil is important in modelling the effects of metal toxicity on the surrounding ecosystem. Current methods based on diffusive gradient thin films (DGTs) and Gel-Integrated Microelectrode are limited in their availability and sensitivity. To address this, S. oneidensis, an anaerobic iron reducing bacterium, was incorporated into a thin layer of agarose to replace the polyacrylamide gel that is normally present in DGT to form biologically mobilizing DGT (BMDGT). Viability analysis revealed that 16-35% of the cells remained viable within the BMDGTs depending on the culturing conditions over a 20 h period with/without metals. Deployment of BMDGTs in standardized metal solutions showed significant differences to cell
free BMDGTs when cells grown in Luria Broth (LB) were incorporated into BMDGTs and deployed under anaerobic conditions. Deployment of these BMDGTs in hematite revealed no significant differences between BMDGTs and BMDGTs containing heat killed cells. Whether heat killed cells retain the ability to affect bioavailability is uncertain. This is the first study to investigate how a microorganism that was incorporated into a DGT device such as the metal reducing bacteria, S. oneidensis, may affect the mobility of metals.
This is the author’s version of a work that was accepted for publication in Chemosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemosphere, 138, 2015 DOI: 10.1016/j.chemosphere.2015.06.018
From: ees.chem.0.31d356.ae2990e7@eesmail.elsevier.com [mailto:ees.chem.0.31d356.ae2990e7@eesmail.elsevier.com] On Behalf Of Chemosphere
Sent: 10 June 2015 04:31
To: Paul Baker; bakerpaulw@gmail.com
Subject: CHEM34688R1 - Editor decision - accepted
Dear Dr. Baker,
I am pleased to inform you that the manuscript "Immobilization of Shewanella oneidensis MR-1 in diffusive gradients in thin films for determining metal bioavailability" (Dr. Paul William Baker) has now been accepted by the editor for publication.
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Chemosphere
