Research output: Contribution to Journal/Magazine › Journal article › peer-review
| <mark>Journal publication date</mark> | 24/07/1998 |
|---|---|
| <mark>Journal</mark> | Journal of Chromatography A |
| Issue number | 1-2 |
| Volume | 814 |
| Number of pages | 6 |
| Pages (from-to) | 105-110 |
| Publication Status | Published |
| <mark>Original language</mark> | English |
The identification and purification of the branch chain hexose hamamelose (2-hydroxymethyl-d-ribose) from plant leaves is described. The procedure includes metabolite extraction in acid and a series of isocratic HPLC separations. Amperometric detection of hamamelose is shown to be quantitative, having a detection limit below 1 nmol. Similar detection of the related compound, 2-carboxy-d-arabinitol, and its monophosphate ester, yields unusually small peaks. Copyright (C) 1998 Elsevier Science B.V.