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  • Gholipourmalekabadi+et+al_2017_Biomed._Mater._10.1088_1748-605X_aa999b

    Rights statement: This is an author-created, un-copyedited version of an article accepted for publication/published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1748-605X/aa999b

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Silk fibroin/amniotic membrane 3D bi-layered artificial skin

Research output: Contribution to journalJournal article

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  • Mazaher Gholipourmalekabadi
  • Ali Samadikuchaksaraei
  • Alexander Seifalian
  • Aleksandra Urbanska
  • Hossein Ghanbarian
  • John George Hardy
  • Mir Omrani
  • Masoud Mozafari
  • Rui Reis
  • Subhas Kundu
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Article number035003
<mark>Journal publication date</mark>20/02/2018
<mark>Journal</mark>Biomedical Materials
Issue number3
Volume13
StatePublished
Early online date10/11/17
Original languageEnglish

Abstract

Burn injury has been reported to be an important cause of morbidity and mortality and it is still considered as unmet clinical need. Although there is a myriad of effective stem cells suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a 3D bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β-sheet transformation as well as to get a tightly coated and inseparable bilayer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate a significant improved mechanical properties of the AM/ESF compared to the AM alone. Both AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against the Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors VEGFa and bFGF when cultured on the AM/ESF for 7 days in comparison with AM alone. The results suggest that AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in clinical setting in skin regeneration.

Bibliographic note

This is an author-created, un-copyedited version of an article accepted for publication/published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1748-605X/aa999b