The Autostereoscopic Multiview 3D Display is robustly developed and widely adopted by both home and professional users however Multiview 3D content generation remains a great challenge. This paper proposes a novel method for Multiview 3D content generation and it describes the necessary steps for Holoscopic 3D image rendering for autostereoscopic Multiview 3D display. The Holoscopic imaging technology mimics the imaging system of insects, such as the fly, utilizing a single camera, equipped with a large number of micro-lenses, to capture a scene, offering rich parallax information and enhanced 3D feeling without the need of wearing specific eyewear. In addition a 3D pixel mapping/conversion algorithm has been developed that is supported by the Multiview 3D display.