The checklist of the plants from the campus of the University of the Amazonía, Florencia, Caquetá, Colombia includes 214 species of vascular plants belonging to 179 genera and 73 families. The families with the most number of species are Fabaceae (19) and Poaceae (10). The wooden species comprise 25.2% of the total, the shrubs 17.2%, grasses 43.9%, epiphytes (including hemiepiphytes) 7.9%, palms 2.3%, vines 2.3%, and hemiparasites 0.9%. The census carried out for trees and bushes yield a total of 1089 individuals, the great majority belonging to introduced species. Despite of being located in an urban center, the university campus of the Universidad de la Amazonia constitutes an important space for the knowledge of the flora.