12,000

We have over 12,000 students, from over 100 countries, within one of the safest campuses in the UK

93%

93% of Lancaster students go into work or further study within six months of graduating

Home > Research > Publications & Outputs > 14-3-3 proteins intact with a 13-lipoxygenase, ...
View graph of relations

« Back

14-3-3 proteins intact with a 13-lipoxygenase, but not with a 9-lipoxygenase.

Research output: Contribution to journalJournal article

Published

  • Wessel L. Holtman
  • Michael R. Roberts
  • Berry J. Oppedijk
  • Christa Testerink
  • Mieke J. Van Zeijl
  • Mei Wang
Journal publication date26/05/2000
JournalFEBS Letters
Journal number1
Volume474
Number of pages5
Pages48-52
Original languageEnglish

Abstract

Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.