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A captured viral interleukin 10 gene with cellular exon structure

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A captured viral interleukin 10 gene with cellular exon structure. / Jayawardane, Gamini; Russell, George C.; Thomson, Jackie et al.
In: Journal of General Virology, Vol. 89, No. 10, 10.2008, p. 2447-2455.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Jayawardane, G, Russell, GC, Thomson, J, Deane, D, Cox, H, Gatherer, D, Ackermann, M, Haig, DM & Stewart, JP 2008, 'A captured viral interleukin 10 gene with cellular exon structure', Journal of General Virology, vol. 89, no. 10, pp. 2447-2455. https://doi.org/10.1099/vir.0.2008/001743-0

APA

Jayawardane, G., Russell, G. C., Thomson, J., Deane, D., Cox, H., Gatherer, D., Ackermann, M., Haig, D. M., & Stewart, J. P. (2008). A captured viral interleukin 10 gene with cellular exon structure. Journal of General Virology, 89(10), 2447-2455. https://doi.org/10.1099/vir.0.2008/001743-0

Vancouver

Jayawardane G, Russell GC, Thomson J, Deane D, Cox H, Gatherer D et al. A captured viral interleukin 10 gene with cellular exon structure. Journal of General Virology. 2008 Oct;89(10):2447-2455. doi: 10.1099/vir.0.2008/001743-0

Author

Jayawardane, Gamini ; Russell, George C. ; Thomson, Jackie et al. / A captured viral interleukin 10 gene with cellular exon structure. In: Journal of General Virology. 2008 ; Vol. 89, No. 10. pp. 2447-2455.

Bibtex

@article{a441a4d4a2d34b079858bc9bf70f9a7e,
title = "A captured viral interleukin 10 gene with cellular exon structure",
abstract = "We have characterized a novel, captured and fully functional viral interleukin (IL)-10 homologue ((OvHV)IL-10) from the gammaherpesvirus ovine herpesvirus 2. Unlike IL-10 homologues from other gammaherpesviruses, the (OvHV)IL-10 peptide sequence was highly divergent from that of the host species. The (OvHV)IL-10 gene is unique amongst virus captured genes in that it has precisely retained the original cellular exon structure, having five exons of similar sizes to the cellular counterparts. However, the sizes of the introns are dramatically reduced. The (OvHV)IL-10 protein was shown to be a non-glycosylated, secreted protein of M(r) 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. Functional assays showed that (OvHV)IL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. This is the first example of a captured herpesvirus gene retaining the full cellular gene structure.",
keywords = "Amino Acid Sequence, Animals, Cattle, Cell Line, Exons, Gammaherpesvirinae, Interleukin-10, Mast Cells, Molecular Sequence Data, Proteins, Sequence Analysis, DNA, Sheep, Viral Proteins",
author = "Gamini Jayawardane and Russell, {George C.} and Jackie Thomson and David Deane and Helen Cox and Derek Gatherer and Mathias Ackermann and Haig, {David M.} and Stewart, {James P.}",
year = "2008",
month = oct,
doi = "10.1099/vir.0.2008/001743-0",
language = "English",
volume = "89",
pages = "2447--2455",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "10",

}

RIS

TY - JOUR

T1 - A captured viral interleukin 10 gene with cellular exon structure

AU - Jayawardane, Gamini

AU - Russell, George C.

AU - Thomson, Jackie

AU - Deane, David

AU - Cox, Helen

AU - Gatherer, Derek

AU - Ackermann, Mathias

AU - Haig, David M.

AU - Stewart, James P.

PY - 2008/10

Y1 - 2008/10

N2 - We have characterized a novel, captured and fully functional viral interleukin (IL)-10 homologue ((OvHV)IL-10) from the gammaherpesvirus ovine herpesvirus 2. Unlike IL-10 homologues from other gammaherpesviruses, the (OvHV)IL-10 peptide sequence was highly divergent from that of the host species. The (OvHV)IL-10 gene is unique amongst virus captured genes in that it has precisely retained the original cellular exon structure, having five exons of similar sizes to the cellular counterparts. However, the sizes of the introns are dramatically reduced. The (OvHV)IL-10 protein was shown to be a non-glycosylated, secreted protein of M(r) 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. Functional assays showed that (OvHV)IL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. This is the first example of a captured herpesvirus gene retaining the full cellular gene structure.

AB - We have characterized a novel, captured and fully functional viral interleukin (IL)-10 homologue ((OvHV)IL-10) from the gammaherpesvirus ovine herpesvirus 2. Unlike IL-10 homologues from other gammaherpesviruses, the (OvHV)IL-10 peptide sequence was highly divergent from that of the host species. The (OvHV)IL-10 gene is unique amongst virus captured genes in that it has precisely retained the original cellular exon structure, having five exons of similar sizes to the cellular counterparts. However, the sizes of the introns are dramatically reduced. The (OvHV)IL-10 protein was shown to be a non-glycosylated, secreted protein of M(r) 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. Functional assays showed that (OvHV)IL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. This is the first example of a captured herpesvirus gene retaining the full cellular gene structure.

KW - Amino Acid Sequence

KW - Animals

KW - Cattle

KW - Cell Line

KW - Exons

KW - Gammaherpesvirinae

KW - Interleukin-10

KW - Mast Cells

KW - Molecular Sequence Data

KW - Proteins

KW - Sequence Analysis, DNA

KW - Sheep

KW - Viral Proteins

U2 - 10.1099/vir.0.2008/001743-0

DO - 10.1099/vir.0.2008/001743-0

M3 - Journal article

C2 - 18796712

VL - 89

SP - 2447

EP - 2455

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 10

ER -