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A dynamic combinatorial screen for novel imine reductase activity.

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A dynamic combinatorial screen for novel imine reductase activity. / Li, Hong; Williams, Paul; Micklefield, Jason et al.
In: Tetrahedron, Vol. 60, No. 3, 12.01.2004, p. 753-758.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Li, H, Williams, P, Micklefield, J, Gardiner, JM & Stephens, G 2004, 'A dynamic combinatorial screen for novel imine reductase activity.', Tetrahedron, vol. 60, no. 3, pp. 753-758. https://doi.org/10.1016/j.tet.2003.10.114

APA

Li, H., Williams, P., Micklefield, J., Gardiner, J. M., & Stephens, G. (2004). A dynamic combinatorial screen for novel imine reductase activity. Tetrahedron, 60(3), 753-758. https://doi.org/10.1016/j.tet.2003.10.114

Vancouver

Li H, Williams P, Micklefield J, Gardiner JM, Stephens G. A dynamic combinatorial screen for novel imine reductase activity. Tetrahedron. 2004 Jan 12;60(3):753-758. doi: 10.1016/j.tet.2003.10.114

Author

Li, Hong ; Williams, Paul ; Micklefield, Jason et al. / A dynamic combinatorial screen for novel imine reductase activity. In: Tetrahedron. 2004 ; Vol. 60, No. 3. pp. 753-758.

Bibtex

@article{e78558e9cc9042b59389d6dde8e1b602,
title = "A dynamic combinatorial screen for novel imine reductase activity.",
abstract = "New imine reductase activity has been discovered in the anaerobic bacterium Acetobacterium woodii by screening a dynamic combinatorial library of virtual imine substrates, using a biphasic water–tetradecane solvent system. Benzylidine aniline and butylidine aniline were reduced to the corresponding amines by caffeate-induced cells, whereas uninduced cells reduced butylidine aniline only. The reductions were detected despite side reactions that consumed some of the starting materials. The new screen can now be extended to discover synthetically useful imine reductases and enzymes that catalyse reactions for which biocatalytic equivalents of the chemical reactions have not yet been discovered.",
keywords = "Virtual library, Imine reductase, Anaerobic biocatalysts",
author = "Hong Li and Paul Williams and Jason Micklefield and Gardiner, {John M.} and Gill Stephens",
year = "2004",
month = jan,
day = "12",
doi = "10.1016/j.tet.2003.10.114",
language = "English",
volume = "60",
pages = "753--758",
journal = "Tetrahedron",
issn = "0040-4020",
publisher = "Elsevier Limited",
number = "3",

}

RIS

TY - JOUR

T1 - A dynamic combinatorial screen for novel imine reductase activity.

AU - Li, Hong

AU - Williams, Paul

AU - Micklefield, Jason

AU - Gardiner, John M.

AU - Stephens, Gill

PY - 2004/1/12

Y1 - 2004/1/12

N2 - New imine reductase activity has been discovered in the anaerobic bacterium Acetobacterium woodii by screening a dynamic combinatorial library of virtual imine substrates, using a biphasic water–tetradecane solvent system. Benzylidine aniline and butylidine aniline were reduced to the corresponding amines by caffeate-induced cells, whereas uninduced cells reduced butylidine aniline only. The reductions were detected despite side reactions that consumed some of the starting materials. The new screen can now be extended to discover synthetically useful imine reductases and enzymes that catalyse reactions for which biocatalytic equivalents of the chemical reactions have not yet been discovered.

AB - New imine reductase activity has been discovered in the anaerobic bacterium Acetobacterium woodii by screening a dynamic combinatorial library of virtual imine substrates, using a biphasic water–tetradecane solvent system. Benzylidine aniline and butylidine aniline were reduced to the corresponding amines by caffeate-induced cells, whereas uninduced cells reduced butylidine aniline only. The reductions were detected despite side reactions that consumed some of the starting materials. The new screen can now be extended to discover synthetically useful imine reductases and enzymes that catalyse reactions for which biocatalytic equivalents of the chemical reactions have not yet been discovered.

KW - Virtual library

KW - Imine reductase

KW - Anaerobic biocatalysts

U2 - 10.1016/j.tet.2003.10.114

DO - 10.1016/j.tet.2003.10.114

M3 - Journal article

VL - 60

SP - 753

EP - 758

JO - Tetrahedron

JF - Tetrahedron

SN - 0040-4020

IS - 3

ER -