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A facile analytical method for reliable selectivity examination in cofactor NADH regeneration

Research output: Contribution to journalJournal article

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  • Tony Saba
  • Joseph W.H. Burnett
  • Jianwei Li
  • Panagiotis N. Kechagiopoulos
  • Xiaodong Wang
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<mark>Journal publication date</mark>25/01/2020
<mark>Journal</mark>Chemical Communications
Issue number8
Volume56
Number of pages4
Pages (from-to)1231-1234
Publication statusPublished
Early online date18/12/19
Original languageEnglish

Abstract

This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.