Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
<mark>Journal publication date</mark> | 05/2014 |
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<mark>Journal</mark> | Biology and Fertility of Soils |
Issue number | 4 |
Volume | 50 |
Number of pages | 10 |
Pages (from-to) | 583-592 |
Publication Status | Published |
Early online date | 8/11/13 |
<mark>Original language</mark> | English |
The aim of this work was to evaluate the treatment of cattle manure with phytases stabilized in allophanic nanoclays as a potential novel phosphorus (P) biofertilization technology for crops grown in volcanic soils (Andisol). Furthermore, because the optimal pH for commercial phytase catalysis does not match the natural pH of manure, a complementary experiment was set up to evaluate the effect of manure inoculation with an alkaline phytase-producing bacterium. Finally, phytase-treated soil, manure, and soil-manure mixtures were evaluated for their P-supplying capacity to wheat plants grown under greenhouse conditions. Treating cattle manure with phytases stabilized in nanoclays resulted in a significant (P a parts per thousand currency signaEuro parts per thousand 0.05) increase of inorganic P in soil extracts (NaOH-EDTA and Olsen). The use of phytase-treated cattle manure increased dry weights by 10 % and the P concentration by 39 % in wheat plants grown under greenhouse conditions, which is equivalent to a P fertilizer rate of about 150 kg of P per hectare. The inoculation of cattle manure with beta-propeller phytase-producing bacteria led to an similar to 10 % increase in inorganic P in the manure extracts. However, applying inoculated manure to soil did not significantly increase wheat yield or P acquisition responses. Our results suggest that the novel approach of incubating cattle manure with phytases stabilized in nanoclay enhances the organic P cycling and P nutrition of plants grown in P-deficient soils.