Home > Research > Publications & Outputs > A yeast-based cytotoxicity and genotoxicity ass...
View graph of relations

A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation.

Research output: Contribution to Journal/MagazineJournal article

Published

Standard

A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation. / Knight, Andrew W.; Keenan, Patrick O.; Goddard, Nicholas J. et al.
In: Journal of Environmental Monitoring, Vol. 6, No. 1, 2004, p. 71-79.

Research output: Contribution to Journal/MagazineJournal article

Harvard

Knight, AW, Keenan, PO, Goddard, NJ, Fielden, PR & Walmsley, RM 2004, 'A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation.', Journal of Environmental Monitoring, vol. 6, no. 1, pp. 71-79. https://doi.org/10.1039/b310206h

APA

Knight, A. W., Keenan, P. O., Goddard, N. J., Fielden, P. R., & Walmsley, R. M. (2004). A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation. Journal of Environmental Monitoring, 6(1), 71-79. https://doi.org/10.1039/b310206h

Vancouver

Knight AW, Keenan PO, Goddard NJ, Fielden PR, Walmsley RM. A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation. Journal of Environmental Monitoring. 2004;6(1):71-79. doi: 10.1039/b310206h

Author

Knight, Andrew W. ; Keenan, Patrick O. ; Goddard, Nicholas J. et al. / A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation. In: Journal of Environmental Monitoring. 2004 ; Vol. 6, No. 1. pp. 71-79.

Bibtex

@article{5f7c18920eea49dea7b77bb230d217c2,
title = "A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation.",
abstract = "An assay capable of simultaneously measuring both general toxicity and more subtle genotoxicity, in aqueous environmental samples, is described. The assay uses eukaryotic (yeast) cells, genetically modified to express a green fluorescent protein (GFP) whenever DNA damage, as a result of exposure to genotoxic agents, is repaired. A measure of the reduction in cell proliferation is used to characterise general toxicity producing familiar EC50 and LOEC data. The assay protocol has been developed for proposed use in the field and hence employs dedicated, portable instrumentation, the development of which is described. A range of environmentally relevant substances has been evaluated using the assay, including solutions of metal ions, solvents and pesticides. Preliminary data comparing the yeast assay's response to that of a standard Daphnia test in the analysis of the toxicity of 34 varied industrial waste effluents are also presented. The sensitivity to a wide range of substances and effluents suggests the assay should be useful for environmental toxicity monitoring.",
author = "Knight, {Andrew W.} and Keenan, {Patrick O.} and Goddard, {Nicholas J.} and Fielden, {Peter R.} and Walmsley, {Richard M.}",
year = "2004",
doi = "10.1039/b310206h",
language = "English",
volume = "6",
pages = "71--79",
journal = "Journal of Environmental Monitoring",
issn = "1464-0325",
publisher = "Royal Society of Chemistry",
number = "1",

}

RIS

TY - JOUR

T1 - A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation.

AU - Knight, Andrew W.

AU - Keenan, Patrick O.

AU - Goddard, Nicholas J.

AU - Fielden, Peter R.

AU - Walmsley, Richard M.

PY - 2004

Y1 - 2004

N2 - An assay capable of simultaneously measuring both general toxicity and more subtle genotoxicity, in aqueous environmental samples, is described. The assay uses eukaryotic (yeast) cells, genetically modified to express a green fluorescent protein (GFP) whenever DNA damage, as a result of exposure to genotoxic agents, is repaired. A measure of the reduction in cell proliferation is used to characterise general toxicity producing familiar EC50 and LOEC data. The assay protocol has been developed for proposed use in the field and hence employs dedicated, portable instrumentation, the development of which is described. A range of environmentally relevant substances has been evaluated using the assay, including solutions of metal ions, solvents and pesticides. Preliminary data comparing the yeast assay's response to that of a standard Daphnia test in the analysis of the toxicity of 34 varied industrial waste effluents are also presented. The sensitivity to a wide range of substances and effluents suggests the assay should be useful for environmental toxicity monitoring.

AB - An assay capable of simultaneously measuring both general toxicity and more subtle genotoxicity, in aqueous environmental samples, is described. The assay uses eukaryotic (yeast) cells, genetically modified to express a green fluorescent protein (GFP) whenever DNA damage, as a result of exposure to genotoxic agents, is repaired. A measure of the reduction in cell proliferation is used to characterise general toxicity producing familiar EC50 and LOEC data. The assay protocol has been developed for proposed use in the field and hence employs dedicated, portable instrumentation, the development of which is described. A range of environmentally relevant substances has been evaluated using the assay, including solutions of metal ions, solvents and pesticides. Preliminary data comparing the yeast assay's response to that of a standard Daphnia test in the analysis of the toxicity of 34 varied industrial waste effluents are also presented. The sensitivity to a wide range of substances and effluents suggests the assay should be useful for environmental toxicity monitoring.

U2 - 10.1039/b310206h

DO - 10.1039/b310206h

M3 - Journal article

VL - 6

SP - 71

EP - 79

JO - Journal of Environmental Monitoring

JF - Journal of Environmental Monitoring

SN - 1464-0325

IS - 1

ER -