Home > Research > Publications & Outputs > Acceleration of gelation and promotion of miner...

Research

Links

Text available via DOI:

Keywords

View graph of relations

Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase. / Douglas, Timothy E. L.; Skwarczynska, Agata; Modrzejewska, Zofia et al.
In: International Journal of Biological Macromolecules, Vol. 56, 05.2013, p. 122-132.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Douglas, TEL, Skwarczynska, A, Modrzejewska, Z, Balcaen, L, Schaubroeck, D, Lycke, S, Vanhaecke, F, Vandenabeele, P, Dubruel, P, Jansen, JA & Leeuwenburgh, SCG 2013, 'Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase', International Journal of Biological Macromolecules, vol. 56, pp. 122-132. https://doi.org/10.1016/j.ijbiomac.2013.02.002

APA

Douglas, T. E. L., Skwarczynska, A., Modrzejewska, Z., Balcaen, L., Schaubroeck, D., Lycke, S., Vanhaecke, F., Vandenabeele, P., Dubruel, P., Jansen, J. A., & Leeuwenburgh, S. C. G. (2013). Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase. International Journal of Biological Macromolecules, 56, 122-132. https://doi.org/10.1016/j.ijbiomac.2013.02.002

Vancouver

Douglas TEL, Skwarczynska A, Modrzejewska Z, Balcaen L, Schaubroeck D, Lycke S et al. Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase. International Journal of Biological Macromolecules. 2013 May;56:122-132. doi: 10.1016/j.ijbiomac.2013.02.002

Author

Douglas, Timothy E. L. ; Skwarczynska, Agata ; Modrzejewska, Zofia et al. / Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase. In: International Journal of Biological Macromolecules. 2013 ; Vol. 56. pp. 122-132.

Bibtex

@article{0aca749fb5bb4532ab220bfc55961e0a,
title = "Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase",
abstract = "Thermosensitive chitosan hydrogels containing sodium beta-glycerophosphate (β-GP), whose gelation is induced by increasing temperature to body temperature, were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone. ALP incorporation led to acceleration of gelation upon increase of temperature for four different chitosan preparations of differing molecular weight, as demonstrated by rheometric time sweeps at 37 °C. Hydrogels containing ALP were subsequently incubated in calcium glycerophosphate (Ca-GP) solution to induce their mineralization with calcium phosphate (CaP) in order to improve their suitability as materials for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP mineral, as confirmed by SEM, FTIR, Raman spectroscopy, XRD, ICP-OES, and increases in dry mass percentage, which rose with increasing ALP concentration and incubation time in Ca-GP solution. The results demonstrate that ALP accelerates formation of thermosensitive chitosan/β-GP hydrogels and induces their mineralization with CaP, which paves the way for applications as injectable bone replacement materials.",
keywords = "Chitosan, Mineralization, Composite, Hydrogel",
author = "Douglas, {Timothy E. L.} and Agata Skwarczynska and Zofia Modrzejewska and Lieve Balcaen and David Schaubroeck and Sylvia Lycke and Frank Vanhaecke and Peter Vandenabeele and Peter Dubruel and Jansen, {John A.} and Leeuwenburgh, {Sander C. G.}",
year = "2013",
month = may,
doi = "10.1016/j.ijbiomac.2013.02.002",
language = "English",
volume = "56",
pages = "122--132",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase

AU - Douglas, Timothy E. L.

AU - Skwarczynska, Agata

AU - Modrzejewska, Zofia

AU - Balcaen, Lieve

AU - Schaubroeck, David

AU - Lycke, Sylvia

AU - Vanhaecke, Frank

AU - Vandenabeele, Peter

AU - Dubruel, Peter

AU - Jansen, John A.

AU - Leeuwenburgh, Sander C. G.

PY - 2013/5

Y1 - 2013/5

N2 - Thermosensitive chitosan hydrogels containing sodium beta-glycerophosphate (β-GP), whose gelation is induced by increasing temperature to body temperature, were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone. ALP incorporation led to acceleration of gelation upon increase of temperature for four different chitosan preparations of differing molecular weight, as demonstrated by rheometric time sweeps at 37 °C. Hydrogels containing ALP were subsequently incubated in calcium glycerophosphate (Ca-GP) solution to induce their mineralization with calcium phosphate (CaP) in order to improve their suitability as materials for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP mineral, as confirmed by SEM, FTIR, Raman spectroscopy, XRD, ICP-OES, and increases in dry mass percentage, which rose with increasing ALP concentration and incubation time in Ca-GP solution. The results demonstrate that ALP accelerates formation of thermosensitive chitosan/β-GP hydrogels and induces their mineralization with CaP, which paves the way for applications as injectable bone replacement materials.

AB - Thermosensitive chitosan hydrogels containing sodium beta-glycerophosphate (β-GP), whose gelation is induced by increasing temperature to body temperature, were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone. ALP incorporation led to acceleration of gelation upon increase of temperature for four different chitosan preparations of differing molecular weight, as demonstrated by rheometric time sweeps at 37 °C. Hydrogels containing ALP were subsequently incubated in calcium glycerophosphate (Ca-GP) solution to induce their mineralization with calcium phosphate (CaP) in order to improve their suitability as materials for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP mineral, as confirmed by SEM, FTIR, Raman spectroscopy, XRD, ICP-OES, and increases in dry mass percentage, which rose with increasing ALP concentration and incubation time in Ca-GP solution. The results demonstrate that ALP accelerates formation of thermosensitive chitosan/β-GP hydrogels and induces their mineralization with CaP, which paves the way for applications as injectable bone replacement materials.

KW - Chitosan

KW - Mineralization

KW - Composite

KW - Hydrogel

U2 - 10.1016/j.ijbiomac.2013.02.002

DO - 10.1016/j.ijbiomac.2013.02.002

M3 - Journal article

VL - 56

SP - 122

EP - 132

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -