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Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum

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Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum. / Twell, David; Patel, Samita; Sorensen, Anna et al.
In: Sexual Plant Reproduction, Vol. 6, No. 4, 10.1993, p. 217-224.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Twell, D, Patel, S, Sorensen, A, Roberts, M, Scott, R, Draper, J & Foster, G 1993, 'Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum', Sexual Plant Reproduction, vol. 6, no. 4, pp. 217-224. https://doi.org/10.1007/BF00231898

APA

Twell, D., Patel, S., Sorensen, A., Roberts, M., Scott, R., Draper, J., & Foster, G. (1993). Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum. Sexual Plant Reproduction, 6(4), 217-224. https://doi.org/10.1007/BF00231898

Vancouver

Twell D, Patel S, Sorensen A, Roberts M, Scott R, Draper J et al. Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum. Sexual Plant Reproduction. 1993 Oct;6(4):217-224. doi: 10.1007/BF00231898

Author

Twell, David ; Patel, Samita ; Sorensen, Anna et al. / Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum. In: Sexual Plant Reproduction. 1993 ; Vol. 6, No. 4. pp. 217-224.

Bibtex

@article{87177ff8c6aa47acaa9d0fd2816e0ba1,
title = "Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum",
abstract = "Seven cytologically distinct stages during micro spore development were identified and used to define the activation of an Arabidopsis anther-specific gene (apg) in transgenic tobacco plants containing an apg promoter-gus fusion. Histochemical analysis of GUS activity showed that the apg promoter was activated in miduninucleate microspores prior to equatorial and polar nuclear migration. Quantitative analysis in isolated spores showed that GUS activity per milligram of protein decreased progressively during pollen development, but on a per spore basis showed a second peak of activity in mid-bicellular pollen. Activation of the apg promoter in isolated gametophytic cells during development was also investigated following DNA delivery by microprojectile bombardment. Levels of transient expression were detectable in uninucleate microspores, but peaked in mid-bicellular pollen, in contrast to the progressive increase in activity of the promoter of the lsquolatersquo pollen gene lat52. These data show that the apg promoter is activated in a biphasic pattern, and indicate that the activity of transcription factors which mediate apg promoter activity persist through microspore mitosis, but decrease during pollen maturation.",
keywords = "Microspore development, Pollen , Transcriptional regulation, transient expression, Microprojectile bombardment, beta-Glucuronidase",
author = "David Twell and Samita Patel and Anna Sorensen and Michael Roberts and Rod Scott and John Draper and Gary Foster",
year = "1993",
month = oct,
doi = "10.1007/BF00231898",
language = "English",
volume = "6",
pages = "217--224",
journal = "Sexual Plant Reproduction",
issn = "0934-0882",
publisher = "Springer Verlag",
number = "4",

}

RIS

TY - JOUR

T1 - Activation and developmental regulation of an Arabidopsis anther-specific promoter in microspores and pollen of Nicotiana tabacum

AU - Twell, David

AU - Patel, Samita

AU - Sorensen, Anna

AU - Roberts, Michael

AU - Scott, Rod

AU - Draper, John

AU - Foster, Gary

PY - 1993/10

Y1 - 1993/10

N2 - Seven cytologically distinct stages during micro spore development were identified and used to define the activation of an Arabidopsis anther-specific gene (apg) in transgenic tobacco plants containing an apg promoter-gus fusion. Histochemical analysis of GUS activity showed that the apg promoter was activated in miduninucleate microspores prior to equatorial and polar nuclear migration. Quantitative analysis in isolated spores showed that GUS activity per milligram of protein decreased progressively during pollen development, but on a per spore basis showed a second peak of activity in mid-bicellular pollen. Activation of the apg promoter in isolated gametophytic cells during development was also investigated following DNA delivery by microprojectile bombardment. Levels of transient expression were detectable in uninucleate microspores, but peaked in mid-bicellular pollen, in contrast to the progressive increase in activity of the promoter of the lsquolatersquo pollen gene lat52. These data show that the apg promoter is activated in a biphasic pattern, and indicate that the activity of transcription factors which mediate apg promoter activity persist through microspore mitosis, but decrease during pollen maturation.

AB - Seven cytologically distinct stages during micro spore development were identified and used to define the activation of an Arabidopsis anther-specific gene (apg) in transgenic tobacco plants containing an apg promoter-gus fusion. Histochemical analysis of GUS activity showed that the apg promoter was activated in miduninucleate microspores prior to equatorial and polar nuclear migration. Quantitative analysis in isolated spores showed that GUS activity per milligram of protein decreased progressively during pollen development, but on a per spore basis showed a second peak of activity in mid-bicellular pollen. Activation of the apg promoter in isolated gametophytic cells during development was also investigated following DNA delivery by microprojectile bombardment. Levels of transient expression were detectable in uninucleate microspores, but peaked in mid-bicellular pollen, in contrast to the progressive increase in activity of the promoter of the lsquolatersquo pollen gene lat52. These data show that the apg promoter is activated in a biphasic pattern, and indicate that the activity of transcription factors which mediate apg promoter activity persist through microspore mitosis, but decrease during pollen maturation.

KW - Microspore development

KW - Pollen

KW - Transcriptional regulation

KW - transient expression

KW - Microprojectile bombardment

KW - beta-Glucuronidase

U2 - 10.1007/BF00231898

DO - 10.1007/BF00231898

M3 - Journal article

VL - 6

SP - 217

EP - 224

JO - Sexual Plant Reproduction

JF - Sexual Plant Reproduction

SN - 0934-0882

IS - 4

ER -