Rights statement: This is the author’s version of a work that was accepted for publication in Environmental and Experimental Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental and Experimental Botany, 176, 2020 DOI: 10.1016/j.envexpbot.2020.104095
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Alternation of wet and dry sides during partial rootzone drying irrigation enhances leaf ethylene evolution
AU - Pérez-Pérez, J.G.
AU - Puertolas, J.
AU - Albacete, A.
AU - Dodd, I.C.
N1 - This is the author’s version of a work that was accepted for publication in Environmental and Experimental Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental and Experimental Botany, 176, 2020 DOI: 10.1016/j.envexpbot.2020.104095
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Soil drying increases endogenous ABA and ACC concentrations in planta, but how these compounds interact to regulate stomatal responses to soil drying and re-watering is still unclear. To determine the temporal dynamics and physiological significance of root, xylem and leaf ABA and ACC concentrations in response to deficit irrigation (DI) or partial rootzone drying (PRD-F) and re-watering, these variables were measured in plants exposed to similar whole pot soil water contents. Both DI and PRD-F plants received only a fraction of the irrigation supplied to well-watered (WW) plants, either to all (DI) or part (PRD-F) of the rootzone of plants grown in split-pots. Both DI and PRD-F induced partial stomatal closure, increased root ABA and ACC accumulation consistent with local soil water content, but did not affect xylem or leaf concentrations of these compounds compared to WW plants. Two hours after re-watering all (DI-RW) or part of the rootzone (PRD-A) to the same soil water content, stomatal conductance returned to WW values or further decreased respectively. Re-watering the whole rootzone had no effect on xylem and leaf ABA and ACC concentrations, while re-watering the dry side of the pot in PRD plants had no effect on xylem and leaf ABA concentrations but increased xylem and leaf ACC concentrations and leaf ethylene evolution. Leaf water potential was similar between all irrigation treatments, with stomatal conductance declining as xylem ABA concentrations and leaf ACC concentrations increased. Prior to re-watering PRD plants, accounting for the spatial differences in soil water uptake best explained variation in xylem ACC concentration suggesting root-to-shoot ACC signalling, but this model did not account for variation in xylem ACC concentration after re-watering the dry side of PRD plants. Thus local (foliar) and long-distance (root-to-shoot) variation in ACC status both seem important in regulating the temporal dynamics of foliar ethylene evolution in plants exposed to PRD. © 2020 Elsevier B.V.
AB - Soil drying increases endogenous ABA and ACC concentrations in planta, but how these compounds interact to regulate stomatal responses to soil drying and re-watering is still unclear. To determine the temporal dynamics and physiological significance of root, xylem and leaf ABA and ACC concentrations in response to deficit irrigation (DI) or partial rootzone drying (PRD-F) and re-watering, these variables were measured in plants exposed to similar whole pot soil water contents. Both DI and PRD-F plants received only a fraction of the irrigation supplied to well-watered (WW) plants, either to all (DI) or part (PRD-F) of the rootzone of plants grown in split-pots. Both DI and PRD-F induced partial stomatal closure, increased root ABA and ACC accumulation consistent with local soil water content, but did not affect xylem or leaf concentrations of these compounds compared to WW plants. Two hours after re-watering all (DI-RW) or part of the rootzone (PRD-A) to the same soil water content, stomatal conductance returned to WW values or further decreased respectively. Re-watering the whole rootzone had no effect on xylem and leaf ABA and ACC concentrations, while re-watering the dry side of the pot in PRD plants had no effect on xylem and leaf ABA concentrations but increased xylem and leaf ACC concentrations and leaf ethylene evolution. Leaf water potential was similar between all irrigation treatments, with stomatal conductance declining as xylem ABA concentrations and leaf ACC concentrations increased. Prior to re-watering PRD plants, accounting for the spatial differences in soil water uptake best explained variation in xylem ACC concentration suggesting root-to-shoot ACC signalling, but this model did not account for variation in xylem ACC concentration after re-watering the dry side of PRD plants. Thus local (foliar) and long-distance (root-to-shoot) variation in ACC status both seem important in regulating the temporal dynamics of foliar ethylene evolution in plants exposed to PRD. © 2020 Elsevier B.V.
KW - ACC
KW - Ethylene
KW - Partial rootzone drying
KW - Root-to-shoot signalling
KW - Soil moisture heterogeneity
U2 - 10.1016/j.envexpbot.2020.104095
DO - 10.1016/j.envexpbot.2020.104095
M3 - Journal article
VL - 176
JO - Environmental and Experimental Botany
JF - Environmental and Experimental Botany
SN - 0098-8472
M1 - 104095
ER -