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An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours.

Research output: Contribution to journalJournal article

Published

Journal publication date03/1999
JournalGlycobiology
Journal number3
Volume9
Number of pages7
Pages285-291
Original languageEnglish

Abstract

A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2- aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub- nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.