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An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours.

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An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours. / Whitham, K. M.; Hadley, J. L.; Morris, H. G. et al.
In: Glycobiology, Vol. 9, No. 3, 03.1999, p. 285-291.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Whitham, KM, Hadley, JL, Morris, HG, Andrew, SM, Nieduszynski, IA & Brown, GM 1999, 'An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours.', Glycobiology, vol. 9, no. 3, pp. 285-291. https://doi.org/10.1093/glycob/9.3.285

APA

Whitham, K. M., Hadley, J. L., Morris, H. G., Andrew, S. M., Nieduszynski, I. A., & Brown, G. M. (1999). An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours. Glycobiology, 9(3), 285-291. https://doi.org/10.1093/glycob/9.3.285

Vancouver

Whitham KM, Hadley JL, Morris HG, Andrew SM, Nieduszynski IA, Brown GM. An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours. Glycobiology. 1999 Mar;9(3):285-291. doi: 10.1093/glycob/9.3.285

Author

Whitham, K. M. ; Hadley, J. L. ; Morris, H. G. et al. / An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours. In: Glycobiology. 1999 ; Vol. 9, No. 3. pp. 285-291.

Bibtex

@article{19782667d061498996ddc0499d6034fe,
title = "An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours.",
abstract = "A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2- aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub- nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.",
author = "Whitham, {K. M.} and Hadley, {J. L.} and Morris, {H. G.} and Andrew, {S. M.} and Nieduszynski, {Ian A.} and Brown, {Gavin M.}",
year = "1999",
month = mar,
doi = "10.1093/glycob/9.3.285",
language = "English",
volume = "9",
pages = "285--291",
journal = "Glycobiology",
issn = "1460-2423",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours.

AU - Whitham, K. M.

AU - Hadley, J. L.

AU - Morris, H. G.

AU - Andrew, S. M.

AU - Nieduszynski, Ian A.

AU - Brown, Gavin M.

PY - 1999/3

Y1 - 1999/3

N2 - A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2- aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub- nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.

AB - A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2- aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub- nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.

U2 - 10.1093/glycob/9.3.285

DO - 10.1093/glycob/9.3.285

M3 - Journal article

VL - 9

SP - 285

EP - 291

JO - Glycobiology

JF - Glycobiology

SN - 1460-2423

IS - 3

ER -