An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera:Symphyta).

Lancaster Environment Centre

Text available via DOI:

https://doi.org/10.1139/W08-067
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Keywords

bacteria, endosymbiont , Hymenoptera , sawflies , Wolbachia

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An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera:Symphyta). / Graham, Robert Iain; Zahner, Vivane; Lucarotti, Christopher.
In: Canadian Journal of Microbiology, Vol. 54, No. 9, 2008, p. 758-768.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{65be32fb33124f6e8240bbc3bf37c029,

title = "An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera:Symphyta).",

abstract = "Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR–DGGE and PCR–RFLP products revealed a dominance of α- and γ-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).",

keywords = "bacteria, endosymbiont , Hymenoptera , sawflies , Wolbachia",

author = "Graham, {Robert Iain} and Vivane Zahner and Christopher Lucarotti",

year = "2008",

doi = "10.1139/W08-067",

language = "English",

volume = "54",

pages = "758--768",

journal = "Canadian Journal of Microbiology",

issn = "0008-4166",

publisher = "National Research Council of Canada",

number = "9",

}

RIS

TY - JOUR

T1 - An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera:Symphyta).

AU - Graham, Robert Iain

AU - Zahner, Vivane

AU - Lucarotti, Christopher

PY - 2008

Y1 - 2008

N2 - Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR–DGGE and PCR–RFLP products revealed a dominance of α- and γ-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).

AB - Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR–DGGE and PCR–RFLP products revealed a dominance of α- and γ-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).

KW - bacteria

KW - endosymbiont

KW - Hymenoptera

KW - sawflies

KW - Wolbachia

U2 - 10.1139/W08-067

DO - 10.1139/W08-067

M3 - Journal article

VL - 54

SP - 758

EP - 768

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 9

ER -

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