An investigation into corneal alkali burns using an organ culture model

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An investigation into corneal alkali burns using an organ culture model. / Zhao, Bojun; Ma, Aihua; Martin, Frank L. et al.
In: Cornea, Vol. 28, No. 5, 06.2009, p. 541-546.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Zhao, B, Ma, A, Martin, FL & Fullwood, NJ 2009, 'An investigation into corneal alkali burns using an organ culture model', Cornea, vol. 28, no. 5, pp. 541-546. https://doi.org/10.1097/ICO.0b013e3181901e08

APA

Zhao, B., Ma, A., Martin, F. L., & Fullwood, N. J. (2009). An investigation into corneal alkali burns using an organ culture model. Cornea, 28(5), 541-546. https://doi.org/10.1097/ICO.0b013e3181901e08

Vancouver

Zhao B, Ma A, Martin FL , Fullwood NJ. An investigation into corneal alkali burns using an organ culture model. Cornea. 2009 Jun;28(5):541-546. doi: 10.1097/ICO.0b013e3181901e08

Author

Zhao, Bojun ; Ma, Aihua ; Martin, Frank L. et al. / An investigation into corneal alkali burns using an organ culture model. In: Cornea. 2009 ; Vol. 28, No. 5. pp. 541-546.

Bibtex

@article{477a7b6e0a0745e892abb8baa1ad3416,

title = "An investigation into corneal alkali burns using an organ culture model",

abstract = "Purpose: To evaluate the usefulness of an in vitro corneal organ culture system for Studying severe alkali hunts.Methods: Fresh bovine cornea was cultured using an established organ culture system. Two molar sodium hydroxide was applied to the corneal and limbal epithelia for 60 seconds. Gross and ultrastructural changes were evaluated at different time points over a 1-week period.Results: The condition of the alkali-burned cornea deteriorated in a time-dependent manner over the 1-week period. Gross changes were evident immediately, and ultrastructural changes were detected in the epithelium, stroma, and endothelium at 1 hour after the alkali burn. By 7 days, most of the corneal and limbal epithelia were destroyed. The corneal stroma was disrupted with separation of lamella and fragmentation of collagen fibrils. By day 7, the endothelium was reduced to cellular debris, although Descemet membrane remained intact.Conclusions: The changes observed in the in vitro organ culture model in response to a severe alkali burn are similar to those observed by other groups in clinical and in vivo studies. We believe that this or similar organ culture systems could be used to evaluate some aspects of severe alkali burns.",

keywords = "corneal organ culture, alkali burns, stem cells, electron microscopy, AMNIOTIC MEMBRANE, RABBIT CORNEA, CELLS, TRANSPLANTATION, EPITHELIUM, MICROSPECTROSCOPY, ULTRASTRUCTURE",

author = "Bojun Zhao and Aihua Ma and Martin, {Frank L.} and Fullwood, {Nigel J.}",

year = "2009",

month = jun,

doi = "10.1097/ICO.0b013e3181901e08",

language = "English",

volume = "28",

pages = "541--546",

journal = "Cornea",

issn = "0277-3740",

publisher = "Lippincott Williams and Wilkins",

number = "5",

}

RIS

TY - JOUR

T1 - An investigation into corneal alkali burns using an organ culture model

AU - Zhao, Bojun

AU - Ma, Aihua

AU - Martin, Frank L.

AU - Fullwood, Nigel J.

PY - 2009/6

Y1 - 2009/6

N2 - Purpose: To evaluate the usefulness of an in vitro corneal organ culture system for Studying severe alkali hunts.Methods: Fresh bovine cornea was cultured using an established organ culture system. Two molar sodium hydroxide was applied to the corneal and limbal epithelia for 60 seconds. Gross and ultrastructural changes were evaluated at different time points over a 1-week period.Results: The condition of the alkali-burned cornea deteriorated in a time-dependent manner over the 1-week period. Gross changes were evident immediately, and ultrastructural changes were detected in the epithelium, stroma, and endothelium at 1 hour after the alkali burn. By 7 days, most of the corneal and limbal epithelia were destroyed. The corneal stroma was disrupted with separation of lamella and fragmentation of collagen fibrils. By day 7, the endothelium was reduced to cellular debris, although Descemet membrane remained intact.Conclusions: The changes observed in the in vitro organ culture model in response to a severe alkali burn are similar to those observed by other groups in clinical and in vivo studies. We believe that this or similar organ culture systems could be used to evaluate some aspects of severe alkali burns.

AB - Purpose: To evaluate the usefulness of an in vitro corneal organ culture system for Studying severe alkali hunts.Methods: Fresh bovine cornea was cultured using an established organ culture system. Two molar sodium hydroxide was applied to the corneal and limbal epithelia for 60 seconds. Gross and ultrastructural changes were evaluated at different time points over a 1-week period.Results: The condition of the alkali-burned cornea deteriorated in a time-dependent manner over the 1-week period. Gross changes were evident immediately, and ultrastructural changes were detected in the epithelium, stroma, and endothelium at 1 hour after the alkali burn. By 7 days, most of the corneal and limbal epithelia were destroyed. The corneal stroma was disrupted with separation of lamella and fragmentation of collagen fibrils. By day 7, the endothelium was reduced to cellular debris, although Descemet membrane remained intact.Conclusions: The changes observed in the in vitro organ culture model in response to a severe alkali burn are similar to those observed by other groups in clinical and in vivo studies. We believe that this or similar organ culture systems could be used to evaluate some aspects of severe alkali burns.

KW - corneal organ culture

KW - alkali burns

KW - stem cells

KW - electron microscopy

KW - AMNIOTIC MEMBRANE

KW - RABBIT CORNEA

KW - CELLS

KW - TRANSPLANTATION

KW - EPITHELIUM

KW - MICROSPECTROSCOPY

KW - ULTRASTRUCTURE

U2 - 10.1097/ICO.0b013e3181901e08

DO - 10.1097/ICO.0b013e3181901e08

M3 - Journal article

VL - 28

SP - 541

EP - 546

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 5

ER -

Research

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