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An investigation into the lipid-binding properties of α-, β- and γ-synucleins in human brain and cerebrospinal fluid

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  • Sultan A Salem
  • David Allsop
  • David M A Mann
  • Takahiko Tokuda
  • Omar M A El-Agnaf
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<mark>Journal publication date</mark>19/09/2007
<mark>Journal</mark>Experimental Brain Research
Volume1170
Number of pages9
Pages (from-to)103-111
Publication statusPublished
Original languageEnglish

Abstract

Parkinson's disease (PD) and dementia with Lewy bodies (DLB) are both characterized by the formation and intraneuronal accumulation of fibrillar aggregates of alpha-synuclein (alpha-syn) protein in affected brain regions. alpha-Syn has biochemical properties and a structural motif characteristic of fatty acid binding proteins. Using the fatty acid binding resin Lipidex-1000, we investigated the capture of alpha-, beta-, and gamma-syn proteins as lipid-associated proteins from normal and DLB brain lysates, and from normal human cerebrospinal fluid (CSF). These were eluted from Lipidex-1000 and analyzed by SDS-NuPAGE followed by Western blotting. Using this methodology, we have been able to extract full-length and truncated forms of alpha-syn from brain lysates. We also extracted low levels of beta-syn from DLB brains, but failed to extract any gamma-syn. We were able to capture only full-length monomeric alpha-syn from normal human CSF. Our data confirm the fatty acid binding properties of alpha-syn, and to a lesser extent beta-syn, but suggest that gamma-syn does not share this same characteristic.