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Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

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Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts. / Kania, D.M.; Ginger, M.L.; Allinson, S.L.
Trypanosomatids. ed. / Paul A.M. Michels; Michael L. Ginger; Dan Zilberstein. New York: Humana Press, 2020. p. 353-364 (Methods in Molecular Biology; Vol. 2116).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Harvard

Kania, DM, Ginger, ML & Allinson, SL 2020, Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts. in PAM Michels, ML Ginger & D Zilberstein (eds), Trypanosomatids. Methods in Molecular Biology, vol. 2116, Humana Press, New York, pp. 353-364. https://doi.org/10.1007/978-1-0716-0294-2_22

APA

Kania, D. M., Ginger, M. L., & Allinson, S. L. (2020). Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts. In P. A. M. Michels, M. L. Ginger, & D. Zilberstein (Eds.), Trypanosomatids (pp. 353-364). (Methods in Molecular Biology; Vol. 2116). Humana Press. https://doi.org/10.1007/978-1-0716-0294-2_22

Vancouver

Kania DM, Ginger ML, Allinson SL. Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts. In Michels PAM, Ginger ML, Zilberstein D, editors, Trypanosomatids. New York: Humana Press. 2020. p. 353-364. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-0294-2_22

Author

Kania, D.M. ; Ginger, M.L. ; Allinson, S.L. / Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts. Trypanosomatids. editor / Paul A.M. Michels ; Michael L. Ginger ; Dan Zilberstein. New York : Humana Press, 2020. pp. 353-364 (Methods in Molecular Biology).

Bibtex

@inbook{3004760156d348a6a7ae64a87a5ebb07,
title = "Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts",
abstract = "Cellular DNA is inherently unstable, subject to both spontaneous hydrolysis and attack by a range of exogenous and endogenous chemicals as well as physical agents such as ionizing and ultraviolet radiation. For parasitic protists, where an inoculum of infectious parasites is typically small and natural infections are often chronic with low parasitemia, they are also vulnerable to DNA damaging agents arising from innate immune defenses. The majority of DNA damage consists of relatively minor changes to the primary structure of the DNA, such as base deamination, oxidation, or alkylation and scission of the phosphodiester backbone. Yet these small changes can have serious consequences, often being mutagenic or cytotoxic. Cells have therefore evolved efficient mechanisms to repair such damage, with base excision and single strand break repair playing the primary role here. In this chapter we describe a method for analyzing the activity from cell extracts of various enzymes involved in the base excision and single strand break repair pathways of trypanosomatid parasites.",
keywords = "AP endonuclease, Base excision repair, Cell extracts, DNA repair, Glycosylase, In vitro repair assay, Leishmania, Polynucleotide kinase/phosphatase, Single-strand break repair, Trypanosoma",
author = "D.M. Kania and M.L. Ginger and S.L. Allinson",
year = "2020",
month = mar,
day = "28",
doi = "10.1007/978-1-0716-0294-2_22",
language = "English",
isbn = "9781071602935",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "353--364",
editor = "Michels, {Paul A.M.} and Ginger, {Michael L.} and Zilberstein, {Dan }",
booktitle = "Trypanosomatids",

}

RIS

TY - CHAP

T1 - Analysis of Base Excision and Single-Strand Break Repair Activities in Trypanosomatid Extracts

AU - Kania, D.M.

AU - Ginger, M.L.

AU - Allinson, S.L.

PY - 2020/3/28

Y1 - 2020/3/28

N2 - Cellular DNA is inherently unstable, subject to both spontaneous hydrolysis and attack by a range of exogenous and endogenous chemicals as well as physical agents such as ionizing and ultraviolet radiation. For parasitic protists, where an inoculum of infectious parasites is typically small and natural infections are often chronic with low parasitemia, they are also vulnerable to DNA damaging agents arising from innate immune defenses. The majority of DNA damage consists of relatively minor changes to the primary structure of the DNA, such as base deamination, oxidation, or alkylation and scission of the phosphodiester backbone. Yet these small changes can have serious consequences, often being mutagenic or cytotoxic. Cells have therefore evolved efficient mechanisms to repair such damage, with base excision and single strand break repair playing the primary role here. In this chapter we describe a method for analyzing the activity from cell extracts of various enzymes involved in the base excision and single strand break repair pathways of trypanosomatid parasites.

AB - Cellular DNA is inherently unstable, subject to both spontaneous hydrolysis and attack by a range of exogenous and endogenous chemicals as well as physical agents such as ionizing and ultraviolet radiation. For parasitic protists, where an inoculum of infectious parasites is typically small and natural infections are often chronic with low parasitemia, they are also vulnerable to DNA damaging agents arising from innate immune defenses. The majority of DNA damage consists of relatively minor changes to the primary structure of the DNA, such as base deamination, oxidation, or alkylation and scission of the phosphodiester backbone. Yet these small changes can have serious consequences, often being mutagenic or cytotoxic. Cells have therefore evolved efficient mechanisms to repair such damage, with base excision and single strand break repair playing the primary role here. In this chapter we describe a method for analyzing the activity from cell extracts of various enzymes involved in the base excision and single strand break repair pathways of trypanosomatid parasites.

KW - AP endonuclease

KW - Base excision repair

KW - Cell extracts

KW - DNA repair

KW - Glycosylase

KW - In vitro repair assay

KW - Leishmania

KW - Polynucleotide kinase/phosphatase

KW - Single-strand break repair

KW - Trypanosoma

U2 - 10.1007/978-1-0716-0294-2_22

DO - 10.1007/978-1-0716-0294-2_22

M3 - Chapter (peer-reviewed)

SN - 9781071602935

T3 - Methods in Molecular Biology

SP - 353

EP - 364

BT - Trypanosomatids

A2 - Michels, Paul A.M.

A2 - Ginger, Michael L.

A2 - Zilberstein, Dan

PB - Humana Press

CY - New York

ER -