Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship. / Dillon, Rod J.; Ivens, Al C.; Churcher, Carol et al.
In: Genomics, Vol. 88, No. 6, 12.2006, p. 831-840.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{1b6918d90c81419eac7a9e52178f913b,

title = "Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship",

abstract = "An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (pelitrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signating pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.",

keywords = "Lutzomyia, expressed sequence tag, Leishmania, genomics, immunity, parasite, midgut, PHLEBOTOMUS-PAPATASI DIPTERA, GLOSSINA-MORSITANS-MORSITANS, MOSQUITO ANOPHELES-GAMBIAE, EXPRESSED SEQUENCE TAGS, AEDES-AEGYPTI, IMMUNE-RESPONSE, MOLECULAR CHARACTERIZATION, PLASMODIUM-BERGHEI, MIDGUT CELLS, LEISHMANIA-MEXICANA",

author = "Dillon, {Rod J.} and Ivens, {Al C.} and Carol Churcher and Nancy Holroyd and Quail, {Michael A.} and Rogers, {Matthew E.} and Soares, {M. Bento} and Bonaldo, {Maria F.} and Casavant, {Thomas L.} and Lehane, {Mike J.} and Bates, {Paul A.}",

year = "2006",

month = dec,

doi = "10.1016/j.ygeno.2006.06.011",

language = "English",

volume = "88",

pages = "831--840",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "6",

}

RIS

TY - JOUR

T1 - Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship

AU - Dillon, Rod J.

AU - Ivens, Al C.

AU - Churcher, Carol

AU - Holroyd, Nancy

AU - Quail, Michael A.

AU - Rogers, Matthew E.

AU - Soares, M. Bento

AU - Bonaldo, Maria F.

AU - Casavant, Thomas L.

AU - Lehane, Mike J.

AU - Bates, Paul A.

PY - 2006/12

Y1 - 2006/12

N2 - An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (pelitrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signating pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.

AB - An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (pelitrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signating pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.

KW - Lutzomyia

KW - expressed sequence tag

KW - Leishmania

KW - genomics

KW - immunity

KW - parasite

KW - midgut

KW - PHLEBOTOMUS-PAPATASI DIPTERA

KW - GLOSSINA-MORSITANS-MORSITANS

KW - MOSQUITO ANOPHELES-GAMBIAE

KW - EXPRESSED SEQUENCE TAGS

KW - AEDES-AEGYPTI

KW - IMMUNE-RESPONSE

KW - MOLECULAR CHARACTERIZATION

KW - PLASMODIUM-BERGHEI

KW - MIDGUT CELLS

KW - LEISHMANIA-MEXICANA

U2 - 10.1016/j.ygeno.2006.06.011

DO - 10.1016/j.ygeno.2006.06.011

M3 - Journal article

C2 - 16887324

VL - 88

SP - 831

EP - 840

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 6

ER -

Research

Links

Text available via DOI:

Keywords