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Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy

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Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy. / Talari, A.C.S.; Raza, A.; Rehman, S. et al.
In: APPLIED SPECTROSCOPY REVIEWS, Vol. 52, No. 10, 01.11.2017, p. 909-924.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Talari ACS, Raza A, Rehman S, Rehman IU. Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy. APPLIED SPECTROSCOPY REVIEWS. 2017 Nov 1;52(10):909-924. Epub 2017 Sept 19. doi: 10.1080/05704928.2017.1363053

Author

Talari, A.C.S. ; Raza, A. ; Rehman, S. et al. / Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy. In: APPLIED SPECTROSCOPY REVIEWS. 2017 ; Vol. 52, No. 10. pp. 909-924.

Bibtex

@article{713e746d2c544607bb83a22eaccda55a,
title = "Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy",
abstract = "Raman spectroscopy is an advanced chemical analytical technique that has gained significant interest in cancer research, in particular early detection and monitoring of cancer, with added advantages of non-invasive and real-time diagnosis. Recently, studies have shown its sensitivity to monitor chemical changes during cancer progression. This information will lead to identification of chemical markers (molecular fingerprints of chemical composition) that can be used as biological markers. In this study, we used a tumor spheroid model that mimics the characteristics of a non-vascular in vitro tumor model, we used a combination of Raman and multivariate approach to identify chemical changes associated with normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroid model. The results provide evidence that lipids, amide I, III and nucleic acid contents differ significantly in normal, hypoxic and necrotic regions. Principal component analysis loading plots has suggested that normal proliferating region separated with low amide I and high-tryptophan content compared to hypoxic and necrotic regions. These differences observed in three regions might be useful in identification of new spectral markers associated stress faced by each region progressing toward necrosis. ",
keywords = "breast cancer, chemometric methods, hypoxia, Raman spectroscopy, spheroid models, Amides, Amino acids, Chemical detection, Diagnosis, Diseases, Nucleic acids, Principal component analysis, Tumors, Breast Cancer, Chemical compositions, Chemometric method, Human breast cancer, Molecular fingerprint, Multivariate approach, Nucleic acid contents, Chemical analysis",
author = "A.C.S. Talari and A. Raza and S. Rehman and I.U. Rehman",
year = "2017",
month = nov,
day = "1",
doi = "10.1080/05704928.2017.1363053",
language = "English",
volume = "52",
pages = "909--924",
journal = "APPLIED SPECTROSCOPY REVIEWS",
issn = "0570-4928",
publisher = "Taylor and Francis Inc.",
number = "10",

}

RIS

TY - JOUR

T1 - Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy

AU - Talari, A.C.S.

AU - Raza, A.

AU - Rehman, S.

AU - Rehman, I.U.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Raman spectroscopy is an advanced chemical analytical technique that has gained significant interest in cancer research, in particular early detection and monitoring of cancer, with added advantages of non-invasive and real-time diagnosis. Recently, studies have shown its sensitivity to monitor chemical changes during cancer progression. This information will lead to identification of chemical markers (molecular fingerprints of chemical composition) that can be used as biological markers. In this study, we used a tumor spheroid model that mimics the characteristics of a non-vascular in vitro tumor model, we used a combination of Raman and multivariate approach to identify chemical changes associated with normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroid model. The results provide evidence that lipids, amide I, III and nucleic acid contents differ significantly in normal, hypoxic and necrotic regions. Principal component analysis loading plots has suggested that normal proliferating region separated with low amide I and high-tryptophan content compared to hypoxic and necrotic regions. These differences observed in three regions might be useful in identification of new spectral markers associated stress faced by each region progressing toward necrosis. 

AB - Raman spectroscopy is an advanced chemical analytical technique that has gained significant interest in cancer research, in particular early detection and monitoring of cancer, with added advantages of non-invasive and real-time diagnosis. Recently, studies have shown its sensitivity to monitor chemical changes during cancer progression. This information will lead to identification of chemical markers (molecular fingerprints of chemical composition) that can be used as biological markers. In this study, we used a tumor spheroid model that mimics the characteristics of a non-vascular in vitro tumor model, we used a combination of Raman and multivariate approach to identify chemical changes associated with normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroid model. The results provide evidence that lipids, amide I, III and nucleic acid contents differ significantly in normal, hypoxic and necrotic regions. Principal component analysis loading plots has suggested that normal proliferating region separated with low amide I and high-tryptophan content compared to hypoxic and necrotic regions. These differences observed in three regions might be useful in identification of new spectral markers associated stress faced by each region progressing toward necrosis. 

KW - breast cancer

KW - chemometric methods

KW - hypoxia

KW - Raman spectroscopy

KW - spheroid models

KW - Amides

KW - Amino acids

KW - Chemical detection

KW - Diagnosis

KW - Diseases

KW - Nucleic acids

KW - Principal component analysis

KW - Tumors

KW - Breast Cancer

KW - Chemical compositions

KW - Chemometric method

KW - Human breast cancer

KW - Molecular fingerprint

KW - Multivariate approach

KW - Nucleic acid contents

KW - Chemical analysis

U2 - 10.1080/05704928.2017.1363053

DO - 10.1080/05704928.2017.1363053

M3 - Journal article

VL - 52

SP - 909

EP - 924

JO - APPLIED SPECTROSCOPY REVIEWS

JF - APPLIED SPECTROSCOPY REVIEWS

SN - 0570-4928

IS - 10

ER -