Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Analyzing normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroids using Raman spectroscopy
AU - Talari, A.C.S.
AU - Raza, A.
AU - Rehman, S.
AU - Rehman, I.U.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Raman spectroscopy is an advanced chemical analytical technique that has gained significant interest in cancer research, in particular early detection and monitoring of cancer, with added advantages of non-invasive and real-time diagnosis. Recently, studies have shown its sensitivity to monitor chemical changes during cancer progression. This information will lead to identification of chemical markers (molecular fingerprints of chemical composition) that can be used as biological markers. In this study, we used a tumor spheroid model that mimics the characteristics of a non-vascular in vitro tumor model, we used a combination of Raman and multivariate approach to identify chemical changes associated with normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroid model. The results provide evidence that lipids, amide I, III and nucleic acid contents differ significantly in normal, hypoxic and necrotic regions. Principal component analysis loading plots has suggested that normal proliferating region separated with low amide I and high-tryptophan content compared to hypoxic and necrotic regions. These differences observed in three regions might be useful in identification of new spectral markers associated stress faced by each region progressing toward necrosis.
AB - Raman spectroscopy is an advanced chemical analytical technique that has gained significant interest in cancer research, in particular early detection and monitoring of cancer, with added advantages of non-invasive and real-time diagnosis. Recently, studies have shown its sensitivity to monitor chemical changes during cancer progression. This information will lead to identification of chemical markers (molecular fingerprints of chemical composition) that can be used as biological markers. In this study, we used a tumor spheroid model that mimics the characteristics of a non-vascular in vitro tumor model, we used a combination of Raman and multivariate approach to identify chemical changes associated with normal proliferating, hypoxic and necrotic regions of T-47D human breast cancer spheroid model. The results provide evidence that lipids, amide I, III and nucleic acid contents differ significantly in normal, hypoxic and necrotic regions. Principal component analysis loading plots has suggested that normal proliferating region separated with low amide I and high-tryptophan content compared to hypoxic and necrotic regions. These differences observed in three regions might be useful in identification of new spectral markers associated stress faced by each region progressing toward necrosis.
KW - breast cancer
KW - chemometric methods
KW - hypoxia
KW - Raman spectroscopy
KW - spheroid models
KW - Amides
KW - Amino acids
KW - Chemical detection
KW - Diagnosis
KW - Diseases
KW - Nucleic acids
KW - Principal component analysis
KW - Tumors
KW - Breast Cancer
KW - Chemical compositions
KW - Chemometric method
KW - Human breast cancer
KW - Molecular fingerprint
KW - Multivariate approach
KW - Nucleic acid contents
KW - Chemical analysis
U2 - 10.1080/05704928.2017.1363053
DO - 10.1080/05704928.2017.1363053
M3 - Journal article
VL - 52
SP - 909
EP - 924
JO - APPLIED SPECTROSCOPY REVIEWS
JF - APPLIED SPECTROSCOPY REVIEWS
SN - 0570-4928
IS - 10
ER -