TY - JOUR

T1 - Apical localization of 1-aminocyclopropane-1-carboxylic acid and its conversion to ethylene in etiolated pea seedlings

AU - Taylor, Jane E

AU - Grosskopf, Deborah A

AU - McGaw, Brian A

AU - Horgan, Roger

AU - Scott, Ian A

PY - 1988/4

Y1 - 1988/4

N2 - The biosynthetic basis for the high rates of ethylene production by the apical region of etiolated pea (Pisum sativum L.) seedlings was investigated. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) was quantified in extracts of various regions of seedlings by measuring isotopic dilution of a 2H-labelled internal standard using selected-ion-monitoring gas chromatography/mass spectrometry. The ACC levels in the apical hook and leaves were much higher than in the expanded internodes of the epicotyl. The capacity of excised tissue sections to convert exogenous ACC to ethylene was also much greater in the apical region, reflecting the distribution of soluble protein in the epicotyl.

AB - The biosynthetic basis for the high rates of ethylene production by the apical region of etiolated pea (Pisum sativum L.) seedlings was investigated. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) was quantified in extracts of various regions of seedlings by measuring isotopic dilution of a 2H-labelled internal standard using selected-ion-monitoring gas chromatography/mass spectrometry. The ACC levels in the apical hook and leaves were much higher than in the expanded internodes of the epicotyl. The capacity of excised tissue sections to convert exogenous ACC to ethylene was also much greater in the apical region, reflecting the distribution of soluble protein in the epicotyl.

KW - 1-Aminocyclopropane-1-carboxylic acid

KW - Ethylene biosynthesis

KW - Pisum (ethylene)

U2 - 10.1007/BF00394882

DO - 10.1007/BF00394882

M3 - Journal article

VL - 174

SP - 112

EP - 114

JO - Planta

JF - Planta

SN - 0032-0935

IS - 1

ER -