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ATR microspectroscopy with multivariate analysis segregates grades of exfoliative cervical cytology.

Research output: Contribution to journalJournal article

Published

Journal publication date5/01/2007
JournalBiochemical and Biophysical Research Communications
Journal number1
Volume352
Number of pages7
Pages213-219
Original languageEnglish

Abstract

Although cervical cancer screening in the UK has led to reductions in the incidence of invasive disease, this programme remains flawed. We set out to examine the potential of infrared (IR) microspectroscopy to allow the profiling of cellular biochemical constituents associated with disease progression. Attenuated total reflection-Fourier Transform IR (ATR) microspectroscopy was employed to interrogate spectral differences between samples of exfoliative cervical cytology collected into liquid based cytology (LBC). These were histologically characterised as normal (n = 5), low-grade (n = 5), high-grade (n = 5) or severe dyskaryosis (? carcinoma) (n = 5). Examination of resultant spectra was coupled with principal component analysis (PCA) and subsequent linear discriminant analysis (LDA). The interrogation of LBC samples using ATR microspectroscopy with PCA–LDA facilitated the discrimination of different categories of exfoliative cytology and allowed the identification of potential biomarkers of abnormality; these occurred prominently in the IR spectral region 1200 cm−1–950 cm−1 consisting of carbohydrates, phosphate, and glycogen. Shifts in the centroids of amide I (≈1650 cm−1) and II (≈1530 cm−1) absorbance bands, indicating conformational changes to the secondary structure of intracellular proteins and associated with increasing disease progression, were also noted. This work demonstrates the potential of ATR microspectroscopy coupled with multivariate analysis to be an objective alternative to routine cytology.