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  • 2016_JHM_PAHs SIP in forest soils

    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Hazardous Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hazardous Materials 308, 2016 DOI: 10.1016/j.jhazmat.2016.01.009

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Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

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Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil. / Song, Mengke; Jiang, Longfei; Zhang, Dayi; Luo, Chunling; Wang, Yan; Yu, Zhiqiang; Yin, Hua; Zhang, Gan.

In: Journal of Hazardous Materials, Vol. 308, 05.05.2016, p. 50-57.

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Song, Mengke ; Jiang, Longfei ; Zhang, Dayi ; Luo, Chunling ; Wang, Yan ; Yu, Zhiqiang ; Yin, Hua ; Zhang, Gan. / Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil. In: Journal of Hazardous Materials. 2016 ; Vol. 308. pp. 50-57.

Bibtex

@article{14170c0fe75e432a8e9a7e1e7d25219a,
title = "Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil",
abstract = "Information on microorganisms possessing the ability to metabolize different polycyclic aromatic hydrocarbons (PAHs) in complex environments helps in understanding PAHs behavior in natural environment and developing bioremediation strategies. In the present study, stable-isotope probing (SIP) was applied to investigate degraders of PAHs in a forest soil with the addition of individually 13C-labeled phenanthrene, anthracene, and fluoranthene. Three distinct phylotypes were identified as the active phenanthrene-, anthracene- and fluoranthene-degrading bacteria. The putative phenanthrene degraders were classified as belonging to the genus Sphingomona. For anthracene, bacteria of the genus Rhodanobacter were the putative degraders, and in the microcosm amended with fluoranthene, the putative degraders were identified as belonging to the phylum Acidobacteria. Our results from DNA-SIP are the first to directly link Rhodanobacter- and Acidobacteria-related bacteria with anthracene and fluoranthene degradation, respectively. The results also illustrate the specificity and diversity of three- and four-ring PAHs degraders in forest soil, contributes to our understanding on natural PAHs biodegradation processes, and also proves the feasibility and practicality of DNA-based SIP for linking functions with identity especially uncultured microorganisms in complex microbial biota.",
keywords = "Anthracene, Biodegradation, DNA stable-isotope probing, Fluoranthene, Forest soil, Phenanthrene",
author = "Mengke Song and Longfei Jiang and Dayi Zhang and Chunling Luo and Yan Wang and Zhiqiang Yu and Hua Yin and Gan Zhang",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Journal of Hazardous Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hazardous Materials 308, 2016 DOI: 10.1016/j.jhazmat.2016.01.009",
year = "2016",
month = may
day = "5",
doi = "10.1016/j.jhazmat.2016.01.009",
language = "English",
volume = "308",
pages = "50--57",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

AU - Song, Mengke

AU - Jiang, Longfei

AU - Zhang, Dayi

AU - Luo, Chunling

AU - Wang, Yan

AU - Yu, Zhiqiang

AU - Yin, Hua

AU - Zhang, Gan

N1 - This is the author’s version of a work that was accepted for publication in Journal of Hazardous Materials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hazardous Materials 308, 2016 DOI: 10.1016/j.jhazmat.2016.01.009

PY - 2016/5/5

Y1 - 2016/5/5

N2 - Information on microorganisms possessing the ability to metabolize different polycyclic aromatic hydrocarbons (PAHs) in complex environments helps in understanding PAHs behavior in natural environment and developing bioremediation strategies. In the present study, stable-isotope probing (SIP) was applied to investigate degraders of PAHs in a forest soil with the addition of individually 13C-labeled phenanthrene, anthracene, and fluoranthene. Three distinct phylotypes were identified as the active phenanthrene-, anthracene- and fluoranthene-degrading bacteria. The putative phenanthrene degraders were classified as belonging to the genus Sphingomona. For anthracene, bacteria of the genus Rhodanobacter were the putative degraders, and in the microcosm amended with fluoranthene, the putative degraders were identified as belonging to the phylum Acidobacteria. Our results from DNA-SIP are the first to directly link Rhodanobacter- and Acidobacteria-related bacteria with anthracene and fluoranthene degradation, respectively. The results also illustrate the specificity and diversity of three- and four-ring PAHs degraders in forest soil, contributes to our understanding on natural PAHs biodegradation processes, and also proves the feasibility and practicality of DNA-based SIP for linking functions with identity especially uncultured microorganisms in complex microbial biota.

AB - Information on microorganisms possessing the ability to metabolize different polycyclic aromatic hydrocarbons (PAHs) in complex environments helps in understanding PAHs behavior in natural environment and developing bioremediation strategies. In the present study, stable-isotope probing (SIP) was applied to investigate degraders of PAHs in a forest soil with the addition of individually 13C-labeled phenanthrene, anthracene, and fluoranthene. Three distinct phylotypes were identified as the active phenanthrene-, anthracene- and fluoranthene-degrading bacteria. The putative phenanthrene degraders were classified as belonging to the genus Sphingomona. For anthracene, bacteria of the genus Rhodanobacter were the putative degraders, and in the microcosm amended with fluoranthene, the putative degraders were identified as belonging to the phylum Acidobacteria. Our results from DNA-SIP are the first to directly link Rhodanobacter- and Acidobacteria-related bacteria with anthracene and fluoranthene degradation, respectively. The results also illustrate the specificity and diversity of three- and four-ring PAHs degraders in forest soil, contributes to our understanding on natural PAHs biodegradation processes, and also proves the feasibility and practicality of DNA-based SIP for linking functions with identity especially uncultured microorganisms in complex microbial biota.

KW - Anthracene

KW - Biodegradation

KW - DNA stable-isotope probing

KW - Fluoranthene

KW - Forest soil

KW - Phenanthrene

U2 - 10.1016/j.jhazmat.2016.01.009

DO - 10.1016/j.jhazmat.2016.01.009

M3 - Journal article

AN - SCOPUS:84955264105

VL - 308

SP - 50

EP - 57

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

ER -