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Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper

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Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper. / Sant'Anna, Mauricio R V; Jones, Nathaniel G; Hindley, Jonathan A; Mendes-Sousa, Antonio F; Dillon, Rod J; Cavalcante, Reginaldo R; Alexander, Bruce; Bates, Paul A.

In: Acta Tropica, Vol. 107, No. 3, 2008, p. 230-237.

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Sant'Anna, Mauricio R V ; Jones, Nathaniel G ; Hindley, Jonathan A ; Mendes-Sousa, Antonio F ; Dillon, Rod J ; Cavalcante, Reginaldo R ; Alexander, Bruce ; Bates, Paul A. / Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper. In: Acta Tropica. 2008 ; Vol. 107, No. 3. pp. 230-237.

Bibtex

@article{8c288c20206b4a6f8c68c9bfb2d94d48,
title = "Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper",
abstract = "The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol described here uses filter-based technology to stabilise and store blood meal DNA, allowing subsequent PCR identification of blood meal sources, as well as parasite detection, in blood-fed sand flies. This technique revealed that 53.6{\%} of field-collected sand flies captured in the back yards of houses in Teresina (Brazil) had fed on chickens. The potential applications of this technique in epidemiological studies and strategic planning for leishmaniasis control programmes are discussed.",
keywords = "Animals, Animals, Domestic, Animals, Wild, Blood, Brazil, Cricetinae, Feeding Behavior, Female, Humans, Leishmaniasis, Visceral, Parasitology, Polymerase Chain Reaction, Psychodidae, Specimen Handling",
author = "Sant'Anna, {Mauricio R V} and Jones, {Nathaniel G} and Hindley, {Jonathan A} and Mendes-Sousa, {Antonio F} and Dillon, {Rod J} and Cavalcante, {Reginaldo R} and Bruce Alexander and Bates, {Paul A}",
year = "2008",
doi = "10.1016/j.actatropica.2008.06.003",
language = "English",
volume = "107",
pages = "230--237",
journal = "Acta Tropica",
issn = "0001-706X",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper

AU - Sant'Anna, Mauricio R V

AU - Jones, Nathaniel G

AU - Hindley, Jonathan A

AU - Mendes-Sousa, Antonio F

AU - Dillon, Rod J

AU - Cavalcante, Reginaldo R

AU - Alexander, Bruce

AU - Bates, Paul A

PY - 2008

Y1 - 2008

N2 - The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol described here uses filter-based technology to stabilise and store blood meal DNA, allowing subsequent PCR identification of blood meal sources, as well as parasite detection, in blood-fed sand flies. This technique revealed that 53.6% of field-collected sand flies captured in the back yards of houses in Teresina (Brazil) had fed on chickens. The potential applications of this technique in epidemiological studies and strategic planning for leishmaniasis control programmes are discussed.

AB - The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol described here uses filter-based technology to stabilise and store blood meal DNA, allowing subsequent PCR identification of blood meal sources, as well as parasite detection, in blood-fed sand flies. This technique revealed that 53.6% of field-collected sand flies captured in the back yards of houses in Teresina (Brazil) had fed on chickens. The potential applications of this technique in epidemiological studies and strategic planning for leishmaniasis control programmes are discussed.

KW - Animals

KW - Animals, Domestic

KW - Animals, Wild

KW - Blood

KW - Brazil

KW - Cricetinae

KW - Feeding Behavior

KW - Female

KW - Humans

KW - Leishmaniasis, Visceral

KW - Parasitology

KW - Polymerase Chain Reaction

KW - Psychodidae

KW - Specimen Handling

U2 - 10.1016/j.actatropica.2008.06.003

DO - 10.1016/j.actatropica.2008.06.003

M3 - Journal article

VL - 107

SP - 230

EP - 237

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

IS - 3

ER -