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Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

Research output: Contribution to journalJournal article

Published
<mark>Journal publication date</mark>08/2009
<mark>Journal</mark>Molecular and Biochemical Parasitology
Issue number2
Volume166
Number of pages3
Pages (from-to)183-185
<mark>State</mark>Published
<mark>Original language</mark>English

Abstract

Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.