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Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

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Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei. / Urbaniak, Michael D.
In: Molecular and Biochemical Parasitology, Vol. 166, No. 2, 08.2009, p. 183-185.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Urbaniak, MD 2009, 'Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei', Molecular and Biochemical Parasitology, vol. 166, no. 2, pp. 183-185. https://doi.org/10.1016/j.molbiopara.2009.03.001

APA

Urbaniak, M. D. (2009). Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei. Molecular and Biochemical Parasitology, 166(2), 183-185. https://doi.org/10.1016/j.molbiopara.2009.03.001

Vancouver

Urbaniak MD. Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei. Molecular and Biochemical Parasitology. 2009 Aug;166(2):183-185. doi: 10.1016/j.molbiopara.2009.03.001

Author

Urbaniak, Michael D. / Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei. In: Molecular and Biochemical Parasitology. 2009 ; Vol. 166, No. 2. pp. 183-185.

Bibtex

@article{1e82cd1ade414772a237818f84705084,
title = "Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei",
abstract = "Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.",
keywords = "Animals, Casein Kinase I, Humans, Kinetics, Molecular Sequence Data, Protein Isoforms, Protozoan Proteins, Recombinant Fusion Proteins, Trypanosoma brucei brucei, Trypanosomiasis, African",
author = "Urbaniak, {Michael D.}",
year = "2009",
month = aug,
doi = "10.1016/j.molbiopara.2009.03.001",
language = "English",
volume = "166",
pages = "183--185",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

AU - Urbaniak, Michael D.

PY - 2009/8

Y1 - 2009/8

N2 - Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.

AB - Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.

KW - Animals

KW - Casein Kinase I

KW - Humans

KW - Kinetics

KW - Molecular Sequence Data

KW - Protein Isoforms

KW - Protozoan Proteins

KW - Recombinant Fusion Proteins

KW - Trypanosoma brucei brucei

KW - Trypanosomiasis, African

U2 - 10.1016/j.molbiopara.2009.03.001

DO - 10.1016/j.molbiopara.2009.03.001

M3 - Journal article

C2 - 19450734

VL - 166

SP - 183

EP - 185

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -