Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra

Lancaster Environment Centre

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https://doi.org/10.1093/jxb/44.1.93
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Keywords

Elder , Sambucus nigra , abscission , polygalacturonase , polyuronides , ethylene

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Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra. / Taylor, Jane E; Webb, Simon TJ; Coupe, Simon A et al.
In: Journal of Experimental Botany, Vol. 44, No. 1, 01.1993, p. 93-98.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{aaf790103ba24045b22706ce55e506a5,

title = "Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra",

abstract = "Leaflet abscission in Sambucus nigra is precipitated by cell wall degradation which is restricted to the site of cell separation. Accompanying wall breakdown is an increase in the activity of the enzyme polygalacturonase (PG) (E.C. 3.2.1.15) and this rise is primarily confined to the abscission zone tissue. The polygalacturonase has a pH optimum of 4·4 and has the characteristics of an endo-acting enzyme. The elevation in enzyme activity is the result of an increase in at least two isoforms of PG as revealed by polyacrylamide gel electrophoresis of the natured protein. Leaflet abscission in S. nigra is associated with an increase in the solubility and depolymerization of polyuronides from the cell wall. These observations are discussed in relation to the mechanism of cell separation during ethylene-stimulated leaf abscission.",

keywords = "Elder , Sambucus nigra , abscission , polygalacturonase , polyuronides , ethylene",

author = "Taylor, {Jane E} and Webb, {Simon TJ} and Coupe, {Simon A} and Tucker, {Gregory A} and Roberts, {Jeremy A}",

year = "1993",

month = jan,

doi = "10.1093/jxb/44.1.93",

language = "English",

volume = "44",

pages = "93--98",

journal = "Journal of Experimental Botany",

issn = "1460-2431",

publisher = "OXFORD UNIV PRESS",

number = "1",

}

RIS

TY - JOUR

T1 - Changes in polygalacturonase activity and solubility of polyuronides during ethylene-stimulated leaf abscission in Sambucus nigra

AU - Taylor, Jane E

AU - Webb, Simon TJ

AU - Coupe, Simon A

AU - Tucker, Gregory A

AU - Roberts, Jeremy A

PY - 1993/1

Y1 - 1993/1

N2 - Leaflet abscission in Sambucus nigra is precipitated by cell wall degradation which is restricted to the site of cell separation. Accompanying wall breakdown is an increase in the activity of the enzyme polygalacturonase (PG) (E.C. 3.2.1.15) and this rise is primarily confined to the abscission zone tissue. The polygalacturonase has a pH optimum of 4·4 and has the characteristics of an endo-acting enzyme. The elevation in enzyme activity is the result of an increase in at least two isoforms of PG as revealed by polyacrylamide gel electrophoresis of the natured protein. Leaflet abscission in S. nigra is associated with an increase in the solubility and depolymerization of polyuronides from the cell wall. These observations are discussed in relation to the mechanism of cell separation during ethylene-stimulated leaf abscission.

AB - Leaflet abscission in Sambucus nigra is precipitated by cell wall degradation which is restricted to the site of cell separation. Accompanying wall breakdown is an increase in the activity of the enzyme polygalacturonase (PG) (E.C. 3.2.1.15) and this rise is primarily confined to the abscission zone tissue. The polygalacturonase has a pH optimum of 4·4 and has the characteristics of an endo-acting enzyme. The elevation in enzyme activity is the result of an increase in at least two isoforms of PG as revealed by polyacrylamide gel electrophoresis of the natured protein. Leaflet abscission in S. nigra is associated with an increase in the solubility and depolymerization of polyuronides from the cell wall. These observations are discussed in relation to the mechanism of cell separation during ethylene-stimulated leaf abscission.

KW - Elder

KW - Sambucus nigra

KW - abscission

KW - polygalacturonase

KW - polyuronides

KW - ethylene

U2 - 10.1093/jxb/44.1.93

DO - 10.1093/jxb/44.1.93

M3 - Journal article

VL - 44

SP - 93

EP - 98

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 1460-2431

IS - 1

ER -

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