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Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra . / Taylor, Jane E; Coupe, Simon A.; Picton, Steve J et al.
In: Plant Molecular Biology, Vol. 24, No. 6, 03.1994, p. 961-964.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Taylor, JE, Coupe, SA, Picton, SJ & Roberts, JA 1994, 'Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra ', Plant Molecular Biology, vol. 24, no. 6, pp. 961-964. https://doi.org/10.1007/BF00014449

APA

Taylor, J. E., Coupe, S. A., Picton, S. J., & Roberts, J. A. (1994). Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra . Plant Molecular Biology, 24(6), 961-964. https://doi.org/10.1007/BF00014449

Vancouver

Taylor JE, Coupe SA, Picton SJ, Roberts JA. Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra . Plant Molecular Biology. 1994 Mar;24(6):961-964. doi: 10.1007/BF00014449

Author

Taylor, Jane E ; Coupe, Simon A. ; Picton, Steve J et al. / Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra . In: Plant Molecular Biology. 1994 ; Vol. 24, No. 6. pp. 961-964.

Bibtex

@article{cb832287844145adae4ffc92b4a458c4,
title = "Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra ",
abstract = "A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission -1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with -1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.",
keywords = "abscission, -1,4-glucanase , cellulase , ethylene , Sambucus nigra",
author = "Taylor, {Jane E} and Coupe, {Simon A.} and Picton, {Steve J} and Roberts, {Jeremy A}",
year = "1994",
month = mar,
doi = "10.1007/BF00014449",
language = "English",
volume = "24",
pages = "961--964",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "6",

}

RIS

TY - JOUR

T1 - Characterisation and accumulation pattern of a mRNA encoding an abscission-related β-1, 4-glucanase from Sambucus nigra

AU - Taylor, Jane E

AU - Coupe, Simon A.

AU - Picton, Steve J

AU - Roberts, Jeremy A

PY - 1994/3

Y1 - 1994/3

N2 - A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission -1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with -1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.

AB - A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission -1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with -1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.

KW - abscission

KW - -1,4-glucanase

KW - cellulase

KW - ethylene

KW - Sambucus nigra

U2 - 10.1007/BF00014449

DO - 10.1007/BF00014449

M3 - Journal article

VL - 24

SP - 961

EP - 964

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 6

ER -