Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose.

Links

http://www.biochemj.org/bj/319/bj3190137.htm

Research output: Contribution to Journal/Magazine › Journal article

Published

Standard

Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose. / Brown, Gavin M.; Huckerby, T. N.; Abram, B. L. et al.
In: Biochemical Journal, Vol. 319, No. 1, 10.1996, p. 137-141.

Research output: Contribution to Journal/Magazine › Journal article

Bibtex

@article{eed27d142de24befac6544a671d44a65,

title = "Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose.",

abstract = "Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-b-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both a(2-3)-linked sialic acid and a(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAca2-3Galb1-4(Fuca1-3)GlcNAcb1-] was identified in this study.",

author = "Brown, {Gavin M.} and Huckerby, {T. N.} and Abram, {B. L.} and Nieduszynski, {I. A.}",

year = "1996",

month = oct,

language = "English",

volume = "319",

pages = "137--141",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

number = "1",

}

RIS

TY - JOUR

T1 - Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose.

AU - Brown, Gavin M.

AU - Huckerby, T. N.

AU - Abram, B. L.

AU - Nieduszynski, I. A.

PY - 1996/10

Y1 - 1996/10

N2 - Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-b-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both a(2-3)-linked sialic acid and a(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAca2-3Galb1-4(Fuca1-3)GlcNAcb1-] was identified in this study.

AB - Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-b-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both a(2-3)-linked sialic acid and a(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAca2-3Galb1-4(Fuca1-3)GlcNAcb1-] was identified in this study.

M3 - Journal article

VL - 319

SP - 137

EP - 141

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -

Research

Links