Home > Research > Publications & Outputs > Characterisation of a second protein encoded by...
View graph of relations

Characterisation of a second protein encoded by the differentially regulated LmcDNA16 gene family of Leishmania major.

Research output: Contribution to journalJournal article

Published
Close
<mark>Journal publication date</mark>04/1997
<mark>Journal</mark>Molecular and Biochemical Parasitology
Issue number2
Volume85
Number of pages11
Pages (from-to)221-231
Publication statusPublished
Original languageEnglish

Abstract

The LmcDNA16 gene family of Leishmania major contains five genes: three highly related sequences, genes A, B and C, and a tandem pair of unrelated sequences, genes D1 and D2. Previous studies have demonstrated that gene B codes for a novel, hydrophilic protein that is present on the surface of infective parasite stages at approximately 10(5) copies per call. This paper describes the identification and characterisation of a second protein encoded by this gene array: the 7.6 kDa A/C protein. This molecule shares considerable amino acid identity with the gene B protein (GBP) but lacks the characteristic proline rich amino acid repeat region. Like GBP, the A/C protein is expressed on the surface of infective metacyclic parasites, despite the lack of conventional signal and anchor sequences. It has previously been suggested that the GBP repetitive sequence plays a role in mediating protein attachment to the parasite surface. It now appears more likely that the conserved amino- and/or carboxyl-terminal domains of the A/C and B proteins are involved in this process.