12,000

We have over 12,000 students, from over 100 countries, within one of the safest campuses in the UK

93%

93% of Lancaster students go into work or further study within six months of graduating

Home > Research > Publications & Outputs > Characterisation of oligosaccharides from the c...
View graph of relations

« Back

Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides.

Research output: Contribution to journalJournal article

Published

Journal publication date03/2001
JournalEuropean Journal of Biochemistry
Journal number5
Volume268
Number of pages9
Pages1181-1189
Original languageEnglish

Abstract

Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-d-galactitol (GalNAc-ol) form. These have the structures: ΔUA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S-ol, ΔUA(β1–3)GalNAc4S(β1–4)l-IdoA(α1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc4S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol and ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, where ΔUA represents a 4,5-unsaturated hexuronic acid (4-deoxy-α-lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete 1H-NMR and 13C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.

Related projects