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Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides.

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Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides. / Huckerby, Thomas N.; Lauder, Robert M.; Brown, Gavin M. et al.
In: European Journal of Biochemistry, Vol. 268, No. 5, 03.2001, p. 1181-1189.

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Huckerby TN, Lauder RM, Brown GM, Nieduszynski IA, Anderson K, Boocock J et al. Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides. European Journal of Biochemistry. 2001 Mar;268(5):1181-1189. doi: 10.1046/j.1432-1327.2001.01948.x

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@article{d82cf336e80b4a83a759ba118ae587f1,
title = "Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides.",
abstract = "Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-d-galactitol (GalNAc-ol) form. These have the structures: ΔUA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S-ol, ΔUA(β1–3)GalNAc4S(β1–4)l-IdoA(α1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc4S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol and ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, where ΔUA represents a 4,5-unsaturated hexuronic acid (4-deoxy-α-lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete 1H-NMR and 13C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.",
author = "Huckerby, {Thomas N.} and Lauder, {Robert M.} and Brown, {Gavin M.} and Nieduszynski, {Ian A.} and Karen Anderson and Joanne Boocock and Sandall, {Patricia L.} and Weeks, {Stephen D.}",
year = "2001",
month = mar,
doi = "10.1046/j.1432-1327.2001.01948.x",
language = "English",
volume = "268",
pages = "1181--1189",
journal = "European Journal of Biochemistry",
issn = "0014-2956",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides.

AU - Huckerby, Thomas N.

AU - Lauder, Robert M.

AU - Brown, Gavin M.

AU - Nieduszynski, Ian A.

AU - Anderson, Karen

AU - Boocock, Joanne

AU - Sandall, Patricia L.

AU - Weeks, Stephen D.

PY - 2001/3

Y1 - 2001/3

N2 - Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-d-galactitol (GalNAc-ol) form. These have the structures: ΔUA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S-ol, ΔUA(β1–3)GalNAc4S(β1–4)l-IdoA(α1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc4S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol and ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, where ΔUA represents a 4,5-unsaturated hexuronic acid (4-deoxy-α-lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete 1H-NMR and 13C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.

AB - Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-d-galactitol (GalNAc-ol) form. These have the structures: ΔUA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S-ol, ΔUA(β1–3)GalNAc4S(β1–4)l-IdoA(α1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc4S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol and ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, where ΔUA represents a 4,5-unsaturated hexuronic acid (4-deoxy-α-lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete 1H-NMR and 13C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.

U2 - 10.1046/j.1432-1327.2001.01948.x

DO - 10.1046/j.1432-1327.2001.01948.x

M3 - Journal article

VL - 268

SP - 1181

EP - 1189

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 5

ER -