Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Characterisation of oligosaccharides from the chondroitin/dermatan sulphates
T2 - 1H and 13C NMR studies of oligosaccharides generated by nitrous acid depolymerisation
AU - Lauder, Robert M.
AU - Huckerby, Thomas
AU - Nieduszynski, Ian
AU - Sadler, Ian H.
PY - 2011/10/18
Y1 - 2011/10/18
N2 - The polymers chondroitin sulphate and dermatan sulphate have been fragmented by an anhydrous hydrazine/nitrous acid procedure. The resulting disaccharides from the polymer repeat sequences were reduced with NaBH4 and purified by ion exchange chromatography. Whereas enzymatic depolymerisation leads to the loss of the distinction between glucuronic and iduronic acids of CS and DS in the resultant disaccharides, nitrous acid depolymerisation retains these structures. Complete 1H and 13C NMR data have been derived for the major components which were shown to have the structures: GlcA(b1?3)- anTal6S-ol (I) and L-IdoA(a1?3)anTal4S-ol (II), where anTal-ol represents (2,5)anhydro-D-talitol and 6S/4S represent O-ester sulphate groups at C-6/C-4 sites.
AB - The polymers chondroitin sulphate and dermatan sulphate have been fragmented by an anhydrous hydrazine/nitrous acid procedure. The resulting disaccharides from the polymer repeat sequences were reduced with NaBH4 and purified by ion exchange chromatography. Whereas enzymatic depolymerisation leads to the loss of the distinction between glucuronic and iduronic acids of CS and DS in the resultant disaccharides, nitrous acid depolymerisation retains these structures. Complete 1H and 13C NMR data have been derived for the major components which were shown to have the structures: GlcA(b1?3)- anTal6S-ol (I) and L-IdoA(a1?3)anTal4S-ol (II), where anTal-ol represents (2,5)anhydro-D-talitol and 6S/4S represent O-ester sulphate groups at C-6/C-4 sites.
KW - Glycosaminoglycan
KW - Chondroitin sulphate/sulfate
KW - NMR spectroscopy
U2 - 10.1016/j.carres.2011.06.033
DO - 10.1016/j.carres.2011.06.033
M3 - Journal article
VL - 346
SP - 2222
EP - 2227
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 1873-426X
IS - 14
ER -