TY - JOUR

T1 - Chitosan hydrogels enriched with polyphenols

T2 - Antibacterial activity, cell adhesion and growth and mineralization

AU - Liskova, Jana

AU - Douglas, Timothy E. L.

AU - Beranova, Jana

AU - Skwarczynska, Agata

AU - Bozic, Mojca

AU - Samal, Sangram Keshari

AU - Modrzejewska, Zofia

AU - Gorgieva, Selestina

AU - Kokol, Vanja

AU - Bacakova, Lucie

PY - 2015/9/20

Y1 - 2015/9/20

N2 - Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na-β-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1 h, 3 h and 6 h incubation and slowed E. coli growth in liquid culture for 150 min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth.

AB - Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na-β-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1 h, 3 h and 6 h incubation and slowed E. coli growth in liquid culture for 150 min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth.

KW - Hydrogel

KW - Polyphenol

KW - Antibacterial

KW - Cytocompatibility

KW - Antioxidant

U2 - 10.1016/j.carbpol.2015.04.043

DO - 10.1016/j.carbpol.2015.04.043

M3 - Journal article

VL - 129

SP - 135

EP - 142

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -