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Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes

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Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes. / Hodges, Matt; Kerns , Jemma; Bentley, Adam J.; Fogarty, Simon; Patel, Imran I.; Martin, Francis L.; Fullwood, Nigel J.

In: ACS Nano, Vol. 5, No. 12, 12.2011, p. 9535-9541.

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Hodges, Matt ; Kerns , Jemma ; Bentley, Adam J. ; Fogarty, Simon ; Patel, Imran I. ; Martin, Francis L. ; Fullwood, Nigel J. / Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes. In: ACS Nano. 2011 ; Vol. 5, No. 12. pp. 9535-9541.

Bibtex

@article{2669fbd9a61a4a2dbaeb8cd4aaa0ef09,
title = "Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes",
abstract = "We applied surface-enhanced Raman spectroscopy (SERS) to immunolabeled endothelial cells to derive enhanced spectra of the biomolecular makeup of the cellular surface. A two-step immunolabeling protocol with gold-conjugated antibodies coupled with silver enhancement to attach silver nanoparticles to the cell surface was employed. This approach generated ∼50-fold SERS enhancement of spectral signals. The SERS spectra exhibited several SERS-enhanced peaks associated with cell membrane components. The SERS detection of silver nanoparticles proved more far more sensitive than conventional light microscopy techniques. The SERS enhancement allowed us to carry out spectral mapping using wavenumbers associated with membrane components that correlated directly with the distribution of silver nanoparticles. SERS has the potential to detect immunolabeling at lower levels than is possible using conventional immunolabeling methods while simultaneously providing unique, spatially defined, biochemical information.",
keywords = "Cell Membrane, Cells, Cultured, Epithelium, Corneal, Epitope Mapping, Humans, Immunohistochemistry, Membrane Proteins, Spectrum Analysis, Raman",
author = "Matt Hodges and Jemma Kerns and Bentley, {Adam J.} and Simon Fogarty and Patel, {Imran I.} and Martin, {Francis L.} and Fullwood, {Nigel J.}",
year = "2011",
month = "12",
doi = "10.1021/nn202652h",
language = "English",
volume = "5",
pages = "9535--9541",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "12",

}

RIS

TY - JOUR

T1 - Combining immunolabeling and surface-enhanced Raman spectroscopy on cell membranes

AU - Hodges, Matt

AU - Kerns , Jemma

AU - Bentley, Adam J.

AU - Fogarty, Simon

AU - Patel, Imran I.

AU - Martin, Francis L.

AU - Fullwood, Nigel J.

PY - 2011/12

Y1 - 2011/12

N2 - We applied surface-enhanced Raman spectroscopy (SERS) to immunolabeled endothelial cells to derive enhanced spectra of the biomolecular makeup of the cellular surface. A two-step immunolabeling protocol with gold-conjugated antibodies coupled with silver enhancement to attach silver nanoparticles to the cell surface was employed. This approach generated ∼50-fold SERS enhancement of spectral signals. The SERS spectra exhibited several SERS-enhanced peaks associated with cell membrane components. The SERS detection of silver nanoparticles proved more far more sensitive than conventional light microscopy techniques. The SERS enhancement allowed us to carry out spectral mapping using wavenumbers associated with membrane components that correlated directly with the distribution of silver nanoparticles. SERS has the potential to detect immunolabeling at lower levels than is possible using conventional immunolabeling methods while simultaneously providing unique, spatially defined, biochemical information.

AB - We applied surface-enhanced Raman spectroscopy (SERS) to immunolabeled endothelial cells to derive enhanced spectra of the biomolecular makeup of the cellular surface. A two-step immunolabeling protocol with gold-conjugated antibodies coupled with silver enhancement to attach silver nanoparticles to the cell surface was employed. This approach generated ∼50-fold SERS enhancement of spectral signals. The SERS spectra exhibited several SERS-enhanced peaks associated with cell membrane components. The SERS detection of silver nanoparticles proved more far more sensitive than conventional light microscopy techniques. The SERS enhancement allowed us to carry out spectral mapping using wavenumbers associated with membrane components that correlated directly with the distribution of silver nanoparticles. SERS has the potential to detect immunolabeling at lower levels than is possible using conventional immunolabeling methods while simultaneously providing unique, spatially defined, biochemical information.

KW - Cell Membrane

KW - Cells, Cultured

KW - Epithelium, Corneal

KW - Epitope Mapping

KW - Humans

KW - Immunohistochemistry

KW - Membrane Proteins

KW - Spectrum Analysis, Raman

UR - http://www.scopus.com/inward/record.url?scp=84555177457&partnerID=8YFLogxK

U2 - 10.1021/nn202652h

DO - 10.1021/nn202652h

M3 - Journal article

C2 - 22067076

VL - 5

SP - 9535

EP - 9541

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 12

ER -