Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis
AU - Dillon, R. J.
AU - Webster, G.
AU - Weightman, A. J.
AU - Dillon, V. M.
AU - Blanford, S.
AU - Charnley, A. K.
PY - 2008/3
Y1 - 2008/3
N2 - The gut bacterial community from four species of feral locusts and grasshoppers was determined by denaturing gradient gel electrophoresis (DGGE) analysis of bacterial 16S rRNA gene fragments. The study revealed an effect of phase polymorphism on gut bacterial diversity in brown locusts from South Africa. A single bacterial phylotype, consistent with Citrobacter sp. dominated the gut microbiota of two sympatric populations of Moroccan and Italian locusts in Spain. There was evidence for Wollbachia sp. in the meadow grasshopper caught locally in the UK. Sequence analysis of DGGE products did not reveal evidence for unculturable bacteria and homologies suggested that bacterial species were principally Gammapoteobacteria from the family Enterobacteriaceae similar to those recorded previously in laboratory reared locusts.
AB - The gut bacterial community from four species of feral locusts and grasshoppers was determined by denaturing gradient gel electrophoresis (DGGE) analysis of bacterial 16S rRNA gene fragments. The study revealed an effect of phase polymorphism on gut bacterial diversity in brown locusts from South Africa. A single bacterial phylotype, consistent with Citrobacter sp. dominated the gut microbiota of two sympatric populations of Moroccan and Italian locusts in Spain. There was evidence for Wollbachia sp. in the meadow grasshopper caught locally in the UK. Sequence analysis of DGGE products did not reveal evidence for unculturable bacteria and homologies suggested that bacterial species were principally Gammapoteobacteria from the family Enterobacteriaceae similar to those recorded previously in laboratory reared locusts.
KW - DGGE
KW - 16S rRNA
KW - locust
KW - bacteria
KW - gut
KW - PARASITE GENOME RELATIONSHIPS
KW - LOCUST SCHISTOCERCA-GREGARIA
KW - MICROBIAL ECOLOGY
KW - DESERT LOCUST
KW - MANAGEMENT REGIMENS
KW - LABORATORY STOCKS
KW - CALEDIA-CAPTIVA
KW - DIVERSITY
KW - HOST
KW - GRASSHOPPERS
U2 - 10.1016/j.jip.2007.09.010
DO - 10.1016/j.jip.2007.09.010
M3 - Journal article
VL - 97
SP - 265
EP - 272
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
SN - 0022-2011
IS - 3
ER -