CYP1B1 expression in prostate is higher in peripheral compared to transition zone.

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CYP1B1 expression in prostate is higher in peripheral compared to transition zone. / Ragavan, N.; Hewitt, R.; Cooper, Leanne J. et al.
In: Cancer Letters, Vol. 215, No. 1, 08.11.2004, p. 69-78.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ragavan, N, Hewitt, R, Cooper, LJ, Ashton, KM, Hindley, AC, Nicholson, CM, Fullwood, NJ, Matanhelia, SS & Martin, FL 2004, 'CYP1B1 expression in prostate is higher in peripheral compared to transition zone.', Cancer Letters, vol. 215, no. 1, pp. 69-78. https://doi.org/10.1016/j.canlet.2004.06.051

APA

Ragavan, N., Hewitt, R., Cooper, L. J., Ashton, K. M., Hindley, A. C., Nicholson, C. M., Fullwood, N. J., Matanhelia, S. S., & Martin, F. L. (2004). CYP1B1 expression in prostate is higher in peripheral compared to transition zone. Cancer Letters, 215(1), 69-78. https://doi.org/10.1016/j.canlet.2004.06.051

Vancouver

Ragavan N, Hewitt R, Cooper LJ, Ashton KM, Hindley AC, Nicholson CM et al. CYP1B1 expression in prostate is higher in peripheral compared to transition zone. Cancer Letters. 2004 Nov 8;215(1):69-78. doi: 10.1016/j.canlet.2004.06.051

Author

Ragavan, N. ; Hewitt, R. ; Cooper, Leanne J. et al. / CYP1B1 expression in prostate is higher in peripheral compared to transition zone. In: Cancer Letters. 2004 ; Vol. 215, No. 1. pp. 69-78.

Bibtex

@article{7370416b1a1a4644a4c1fe5070cb6953,

title = "CYP1B1 expression in prostate is higher in peripheral compared to transition zone.",

abstract = "Prostate cancer (CaP) mostly occurs in the peripheral zone whereas benign prostatic hypertrophy (BPH) occurs in the transition zone. Human prostates (n=12) were obtained, with ethical approval, from radical retropubic prostatectomies. Following resection, tissue sets consisting of peripheral zone and transition zone were isolated from a lobe pre-operatively identified as negative for CaP. Real-time RT-PCR was employed to quantitatively examine CYP1A1, CYP1A2 and CYP1B1. Quantifiable CYP1A1 expression was observed (in nine out of twelve tissue sets) whilst CYP1A2 mRNA transcripts, although detectable (in six out of twelve tissue sets), were unquantifiable. In ten tissue sets, 2- to 6-fold higher CYP1B1 expression in peripheral zone as compared to transition zone was observed. In the other two, equal CYP1B1 expression levels were observed; retrospective examination identified malignancy in one of the zones. Inter-individual variations (up to 10-fold) in CYP1B1 were also noted. Immunohistochemistry for CYP1B1 showed epithelial and stromal nuclear staining. Since CYP1B1 metabolises hormones and carcinogens our results, if confirmed, suggest that this enzyme may influence susceptibility to CaP.",

keywords = "CYP1B1, Human prostate, Nuclear staining, Peripheral zone, Radical retropubic prostatectomy, Transition zone",

author = "N. Ragavan and R. Hewitt and Cooper, {Leanne J.} and Ashton, {K. M.} and Hindley, {A. C.} and Nicholson, {C. M.} and Fullwood, {Nigel J.} and Matanhelia, {S. S.} and Martin, {F. L.}",

year = "2004",

month = nov,

day = "8",

doi = "10.1016/j.canlet.2004.06.051",

language = "English",

volume = "215",

pages = "69--78",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

RIS

TY - JOUR

T1 - CYP1B1 expression in prostate is higher in peripheral compared to transition zone.

AU - Ragavan, N.

AU - Hewitt, R.

AU - Cooper, Leanne J.

AU - Ashton, K. M.

AU - Hindley, A. C.

AU - Nicholson, C. M.

AU - Fullwood, Nigel J.

AU - Matanhelia, S. S.

AU - Martin, F. L.

PY - 2004/11/8

Y1 - 2004/11/8

N2 - Prostate cancer (CaP) mostly occurs in the peripheral zone whereas benign prostatic hypertrophy (BPH) occurs in the transition zone. Human prostates (n=12) were obtained, with ethical approval, from radical retropubic prostatectomies. Following resection, tissue sets consisting of peripheral zone and transition zone were isolated from a lobe pre-operatively identified as negative for CaP. Real-time RT-PCR was employed to quantitatively examine CYP1A1, CYP1A2 and CYP1B1. Quantifiable CYP1A1 expression was observed (in nine out of twelve tissue sets) whilst CYP1A2 mRNA transcripts, although detectable (in six out of twelve tissue sets), were unquantifiable. In ten tissue sets, 2- to 6-fold higher CYP1B1 expression in peripheral zone as compared to transition zone was observed. In the other two, equal CYP1B1 expression levels were observed; retrospective examination identified malignancy in one of the zones. Inter-individual variations (up to 10-fold) in CYP1B1 were also noted. Immunohistochemistry for CYP1B1 showed epithelial and stromal nuclear staining. Since CYP1B1 metabolises hormones and carcinogens our results, if confirmed, suggest that this enzyme may influence susceptibility to CaP.

AB - Prostate cancer (CaP) mostly occurs in the peripheral zone whereas benign prostatic hypertrophy (BPH) occurs in the transition zone. Human prostates (n=12) were obtained, with ethical approval, from radical retropubic prostatectomies. Following resection, tissue sets consisting of peripheral zone and transition zone were isolated from a lobe pre-operatively identified as negative for CaP. Real-time RT-PCR was employed to quantitatively examine CYP1A1, CYP1A2 and CYP1B1. Quantifiable CYP1A1 expression was observed (in nine out of twelve tissue sets) whilst CYP1A2 mRNA transcripts, although detectable (in six out of twelve tissue sets), were unquantifiable. In ten tissue sets, 2- to 6-fold higher CYP1B1 expression in peripheral zone as compared to transition zone was observed. In the other two, equal CYP1B1 expression levels were observed; retrospective examination identified malignancy in one of the zones. Inter-individual variations (up to 10-fold) in CYP1B1 were also noted. Immunohistochemistry for CYP1B1 showed epithelial and stromal nuclear staining. Since CYP1B1 metabolises hormones and carcinogens our results, if confirmed, suggest that this enzyme may influence susceptibility to CaP.

KW - CYP1B1

KW - Human prostate

KW - Nuclear staining

KW - Peripheral zone

KW - Radical retropubic prostatectomy

KW - Transition zone

U2 - 10.1016/j.canlet.2004.06.051

DO - 10.1016/j.canlet.2004.06.051

M3 - Journal article

VL - 215

SP - 69

EP - 78

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -

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