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Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms. / Igamberdiev, Abir U.; Ivlev, Alexander A.; Bykova, Natalia V. et al.
In: Photosynthesis Research, Vol. 67, No. 3, 03.2001, p. 177-184.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Igamberdiev, AU, Ivlev, AA, Bykova, NV, Threlkeld, CN, Lea, PJ & Gardeström, P 2001, 'Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms.', Photosynthesis Research, vol. 67, no. 3, pp. 177-184. https://doi.org/10.1023/A:1010635308668

APA

Igamberdiev, A. U., Ivlev, A. A., Bykova, N. V., Threlkeld, C. N., Lea, P. J., & Gardeström, P. (2001). Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms. Photosynthesis Research, 67(3), 177-184. https://doi.org/10.1023/A:1010635308668

Vancouver

Igamberdiev AU, Ivlev AA, Bykova NV, Threlkeld CN, Lea PJ, Gardeström P. Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms. Photosynthesis Research. 2001 Mar;67(3):177-184. doi: 10.1023/A:1010635308668

Author

Igamberdiev, Abir U. ; Ivlev, Alexander A. ; Bykova, Natalia V. et al. / Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms. In: Photosynthesis Research. 2001 ; Vol. 67, No. 3. pp. 177-184.

Bibtex

@article{a2a06d3f5b844c1a8afc1506c5aee93c,
title = "Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms.",
abstract = "Carbon isotope effects were investigated for the reaction catalyzed by the glycine decarboxylase complex (GDC; EC 2.1.2.10). Mitochondria isolated from leaves of pea (Pisum sativum L.) and spinach (Spinacia oleracea L.) were incubated with glycine, and the CO2 evolved was analyzed for the carbon isotope ratio (δ13C). Within the range of parameters tested (temperature, pH, combination of cofactors NAD+, ADP, pyridoxal 5-phosphate), carbon isotope shifts of CO2 relative to the C1-carboxyl carbon of glycine varied from +14‰ to −7‰. The maximum effect of cofactors was observed for NAD+, the removal of which resulted in a strong 12C enrichment of the CO2 evolved. This indicates the possibility of isotope effects with both positive and negative signs in the GDC reaction. The measurement of δ13C in the leaves of the GDC-deficient barley (Hordeum vulgare L.) mutant (LaPr 87/30) plants indicated that photorespiratory carbon isotope fractionation, opposite in sign when compared to the carbon isotope effect during CO2 photoassimilation, takes place in vivo. Thus the key reaction of photorespiration catalyzed by GDC, together with the key reaction of CO2 fixation catalyzed by ribulose-1,5-bisphosphate carboxylase, both contribute to carbon isotope fractionation in photosynthesis.",
keywords = "carbon isotope fractionation - glycine decarboxylase - photorespiration - photorespiratory mutants - plant mitochondria",
author = "Igamberdiev, {Abir U.} and Ivlev, {Alexander A.} and Bykova, {Natalia V.} and Threlkeld, {Charles N.} and Lea, {Peter J.} and Per Gardestr{\"o}m",
year = "2001",
month = mar,
doi = "10.1023/A:1010635308668",
language = "English",
volume = "67",
pages = "177--184",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "3",

}

RIS

TY - JOUR

T1 - Decarboxylation of glycine contributes to carbon isotope fractionation in photosynthetic organisms.

AU - Igamberdiev, Abir U.

AU - Ivlev, Alexander A.

AU - Bykova, Natalia V.

AU - Threlkeld, Charles N.

AU - Lea, Peter J.

AU - Gardeström, Per

PY - 2001/3

Y1 - 2001/3

N2 - Carbon isotope effects were investigated for the reaction catalyzed by the glycine decarboxylase complex (GDC; EC 2.1.2.10). Mitochondria isolated from leaves of pea (Pisum sativum L.) and spinach (Spinacia oleracea L.) were incubated with glycine, and the CO2 evolved was analyzed for the carbon isotope ratio (δ13C). Within the range of parameters tested (temperature, pH, combination of cofactors NAD+, ADP, pyridoxal 5-phosphate), carbon isotope shifts of CO2 relative to the C1-carboxyl carbon of glycine varied from +14‰ to −7‰. The maximum effect of cofactors was observed for NAD+, the removal of which resulted in a strong 12C enrichment of the CO2 evolved. This indicates the possibility of isotope effects with both positive and negative signs in the GDC reaction. The measurement of δ13C in the leaves of the GDC-deficient barley (Hordeum vulgare L.) mutant (LaPr 87/30) plants indicated that photorespiratory carbon isotope fractionation, opposite in sign when compared to the carbon isotope effect during CO2 photoassimilation, takes place in vivo. Thus the key reaction of photorespiration catalyzed by GDC, together with the key reaction of CO2 fixation catalyzed by ribulose-1,5-bisphosphate carboxylase, both contribute to carbon isotope fractionation in photosynthesis.

AB - Carbon isotope effects were investigated for the reaction catalyzed by the glycine decarboxylase complex (GDC; EC 2.1.2.10). Mitochondria isolated from leaves of pea (Pisum sativum L.) and spinach (Spinacia oleracea L.) were incubated with glycine, and the CO2 evolved was analyzed for the carbon isotope ratio (δ13C). Within the range of parameters tested (temperature, pH, combination of cofactors NAD+, ADP, pyridoxal 5-phosphate), carbon isotope shifts of CO2 relative to the C1-carboxyl carbon of glycine varied from +14‰ to −7‰. The maximum effect of cofactors was observed for NAD+, the removal of which resulted in a strong 12C enrichment of the CO2 evolved. This indicates the possibility of isotope effects with both positive and negative signs in the GDC reaction. The measurement of δ13C in the leaves of the GDC-deficient barley (Hordeum vulgare L.) mutant (LaPr 87/30) plants indicated that photorespiratory carbon isotope fractionation, opposite in sign when compared to the carbon isotope effect during CO2 photoassimilation, takes place in vivo. Thus the key reaction of photorespiration catalyzed by GDC, together with the key reaction of CO2 fixation catalyzed by ribulose-1,5-bisphosphate carboxylase, both contribute to carbon isotope fractionation in photosynthesis.

KW - carbon isotope fractionation - glycine decarboxylase - photorespiration - photorespiratory mutants - plant mitochondria

U2 - 10.1023/A:1010635308668

DO - 10.1023/A:1010635308668

M3 - Journal article

VL - 67

SP - 177

EP - 184

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 3

ER -