Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Decreased α-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson’s disease.
AU - Tokuda, Takahiko
AU - Salem, Sultan A.
AU - Allsop, David
AU - Mizuno, Toshiki
AU - Nakagawa, Masanori
AU - Qureshi, Mohamed M.
AU - Locascio, Joseph J.
AU - Schlossmacher, Michael G.
AU - El-Agnaf, Omar
PY - 2006/10/13
Y1 - 2006/10/13
N2 - There is ample biochemical, pathological, and genetic evidence that the metabolism of -synuclein (-syn) plays a crucial role in the pathogenesis of Parkinson disease (PD). To examine whether quantification of -syn in cerebrospinal fluid (CSF) is potentially informative in the diagnosis of PD, we developed a specific ELISA system and measured the concentration of -syn in CSF from 33 patients with PD (diagnosed according to UK PD Society Brain Bank criteria) and 38 control subjects including 9 neurologically healthy individuals. We found that PD patients had significantly lower -syn levels in their CSF than the control groups (p < 0.0001) even after adjusting for gender and age. Age was independently associated with lower -syn levels. Logistic regression analysis showed that reduction in CSF -syn served as a significant predictor of PD beyond age and gender alone (area under ROC curve, c = 0.882). Furthermore, we observed a close inverse correlation between -syn levels in CSF and assigned Hoehn and Yahr score in this cohort of 71 living subjects (p < 0.0001), even after adjusting for age. These findings identify in the quantification of -syn from CSF a potential laboratory marker to aid the clinical diagnosis of PD.
AB - There is ample biochemical, pathological, and genetic evidence that the metabolism of -synuclein (-syn) plays a crucial role in the pathogenesis of Parkinson disease (PD). To examine whether quantification of -syn in cerebrospinal fluid (CSF) is potentially informative in the diagnosis of PD, we developed a specific ELISA system and measured the concentration of -syn in CSF from 33 patients with PD (diagnosed according to UK PD Society Brain Bank criteria) and 38 control subjects including 9 neurologically healthy individuals. We found that PD patients had significantly lower -syn levels in their CSF than the control groups (p < 0.0001) even after adjusting for gender and age. Age was independently associated with lower -syn levels. Logistic regression analysis showed that reduction in CSF -syn served as a significant predictor of PD beyond age and gender alone (area under ROC curve, c = 0.882). Furthermore, we observed a close inverse correlation between -syn levels in CSF and assigned Hoehn and Yahr score in this cohort of 71 living subjects (p < 0.0001), even after adjusting for age. These findings identify in the quantification of -syn from CSF a potential laboratory marker to aid the clinical diagnosis of PD.
KW - Parkinson disease
KW - Neurodegenerative diseases
KW - -Synuclein
KW - Cerebrospinal fluid
KW - ELISA
KW - Biomarker
KW - Diagnosis
U2 - 10.1016/j.bbrc.2006.08.024
DO - 10.1016/j.bbrc.2006.08.024
M3 - Journal article
VL - 349
SP - 162
EP - 166
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -