Home > Research > Publications & Outputs > Defining the Identity and Dynamics of Adult Gas...

Links

Text available via DOI:

View graph of relations

Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells

Research output: Contribution to journalJournal article

Published
  • Seungmin Han
  • Juergen Fink
  • David J Jörg
  • Eunmin Lee
  • Min Kyu Yum
  • Lemonia Chatzeli
  • Sebastian R Merker
  • Manon Josserand
  • Teodora Trendafilova
  • Amanda Andersson-Rolf
  • Catherine Dabrowska
  • Hyunki Kim
  • Ronald Naumann
  • Ji-Hyun Lee
  • Nobuo Sasaki
  • Onur Basak
  • Hans Clevers
  • Daniel E Stange
  • Anna Philpott
  • Jong Kyoung Kim
  • Benjamin D Simons
  • Bon-Kyoung Koo
Close
<mark>Journal publication date</mark>5/09/2019
<mark>Journal</mark>Cell Stem Cell
Issue number3
Volume25
Number of pages15
Pages (from-to)342-356.e7
Publication statusPublished
Early online date15/08/19
Original languageEnglish

Abstract

The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.