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Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells

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Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells. / Han, Seungmin; Fink, Juergen; Jörg, David J; Lee, Eunmin; Yum, Min Kyu; Chatzeli, Lemonia; Merker, Sebastian R; Josserand, Manon; Trendafilova, Teodora; Andersson-Rolf, Amanda; Dabrowska, Catherine; Kim, Hyunki; Naumann, Ronald; Lee, Ji-Hyun; Sasaki, Nobuo; Mort, Richard Lester; Basak, Onur; Clevers, Hans; Stange, Daniel E; Philpott, Anna; Kim, Jong Kyoung; Simons, Benjamin D; Koo, Bon-Kyoung.

In: Cell Stem Cell, Vol. 25, No. 3, 05.09.2019, p. 342-356.e7.

Research output: Contribution to journalJournal article

Harvard

Han, S, Fink, J, Jörg, DJ, Lee, E, Yum, MK, Chatzeli, L, Merker, SR, Josserand, M, Trendafilova, T, Andersson-Rolf, A, Dabrowska, C, Kim, H, Naumann, R, Lee, J-H, Sasaki, N, Mort, RL, Basak, O, Clevers, H, Stange, DE, Philpott, A, Kim, JK, Simons, BD & Koo, B-K 2019, 'Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells', Cell Stem Cell, vol. 25, no. 3, pp. 342-356.e7. https://doi.org/10.1016/j.stem.2019.07.008

APA

Han, S., Fink, J., Jörg, D. J., Lee, E., Yum, M. K., Chatzeli, L., ... Koo, B-K. (2019). Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells. Cell Stem Cell, 25(3), 342-356.e7. https://doi.org/10.1016/j.stem.2019.07.008

Vancouver

Han S, Fink J, Jörg DJ, Lee E, Yum MK, Chatzeli L et al. Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells. Cell Stem Cell. 2019 Sep 5;25(3):342-356.e7. https://doi.org/10.1016/j.stem.2019.07.008

Author

Han, Seungmin ; Fink, Juergen ; Jörg, David J ; Lee, Eunmin ; Yum, Min Kyu ; Chatzeli, Lemonia ; Merker, Sebastian R ; Josserand, Manon ; Trendafilova, Teodora ; Andersson-Rolf, Amanda ; Dabrowska, Catherine ; Kim, Hyunki ; Naumann, Ronald ; Lee, Ji-Hyun ; Sasaki, Nobuo ; Mort, Richard Lester ; Basak, Onur ; Clevers, Hans ; Stange, Daniel E ; Philpott, Anna ; Kim, Jong Kyoung ; Simons, Benjamin D ; Koo, Bon-Kyoung. / Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells. In: Cell Stem Cell. 2019 ; Vol. 25, No. 3. pp. 342-356.e7.

Bibtex

@article{2f6dccfdf57447fa939c05b69498301d,
title = "Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells",
abstract = "The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of {"}punctuated{"} neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.",
keywords = "gastric corpus isthmus stem cell, two stem cell compartments, punctuated neutral drift, unbiased genetic labeling, deep tissue imaging, biophysical modeling, single-cell RNA-seq, Lgr5, Troy, intestine",
author = "Seungmin Han and Juergen Fink and J{\"o}rg, {David J} and Eunmin Lee and Yum, {Min Kyu} and Lemonia Chatzeli and Merker, {Sebastian R} and Manon Josserand and Teodora Trendafilova and Amanda Andersson-Rolf and Catherine Dabrowska and Hyunki Kim and Ronald Naumann and Ji-Hyun Lee and Nobuo Sasaki and Mort, {Richard Lester} and Onur Basak and Hans Clevers and Stange, {Daniel E} and Anna Philpott and Kim, {Jong Kyoung} and Simons, {Benjamin D} and Bon-Kyoung Koo",
year = "2019",
month = "9",
day = "5",
doi = "10.1016/j.stem.2019.07.008",
language = "English",
volume = "25",
pages = "342--356.e7",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells

AU - Han, Seungmin

AU - Fink, Juergen

AU - Jörg, David J

AU - Lee, Eunmin

AU - Yum, Min Kyu

AU - Chatzeli, Lemonia

AU - Merker, Sebastian R

AU - Josserand, Manon

AU - Trendafilova, Teodora

AU - Andersson-Rolf, Amanda

AU - Dabrowska, Catherine

AU - Kim, Hyunki

AU - Naumann, Ronald

AU - Lee, Ji-Hyun

AU - Sasaki, Nobuo

AU - Mort, Richard Lester

AU - Basak, Onur

AU - Clevers, Hans

AU - Stange, Daniel E

AU - Philpott, Anna

AU - Kim, Jong Kyoung

AU - Simons, Benjamin D

AU - Koo, Bon-Kyoung

PY - 2019/9/5

Y1 - 2019/9/5

N2 - The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.

AB - The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.

KW - gastric corpus isthmus stem cell

KW - two stem cell compartments

KW - punctuated neutral drift

KW - unbiased genetic labeling

KW - deep tissue imaging

KW - biophysical modeling

KW - single-cell RNA-seq

KW - Lgr5

KW - Troy

KW - intestine

U2 - 10.1016/j.stem.2019.07.008

DO - 10.1016/j.stem.2019.07.008

M3 - Journal article

C2 - 31422913

VL - 25

SP - 342-356.e7

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 3

ER -