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  • Hathaway et al 2019

    Rights statement: This is an author-created, un-copyedited version of an article accepted for publication/published in Journal of Physics D: Applied Physics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1361-6463/ab4539

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Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material

Research output: Contribution to journalJournal article

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Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material. / Hathaway, H.J.; Patenall, B.L.; Thet, N.T.; Sedgwick, A.C.; Williams, G.T.; Jenkins, A.T.A.; Allinson, S.L.; Short, R.D.

In: Journal of Physics D: Applied Physics, Vol. 52, No. 50, 505203, 09.10.2019.

Research output: Contribution to journalJournal article

Harvard

Hathaway, HJ, Patenall, BL, Thet, NT, Sedgwick, AC, Williams, GT, Jenkins, ATA, Allinson, SL & Short, RD 2019, 'Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material', Journal of Physics D: Applied Physics, vol. 52, no. 50, 505203. https://doi.org/10.1088/1361-6463/ab4539

APA

Hathaway, H. J., Patenall, B. L., Thet, N. T., Sedgwick, A. C., Williams, G. T., Jenkins, A. T. A., ... Short, R. D. (2019). Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material. Journal of Physics D: Applied Physics, 52(50), [505203]. https://doi.org/10.1088/1361-6463/ab4539

Vancouver

Hathaway HJ, Patenall BL, Thet NT, Sedgwick AC, Williams GT, Jenkins ATA et al. Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material. Journal of Physics D: Applied Physics. 2019 Oct 9;52(50). 505203. https://doi.org/10.1088/1361-6463/ab4539

Author

Hathaway, H.J. ; Patenall, B.L. ; Thet, N.T. ; Sedgwick, A.C. ; Williams, G.T. ; Jenkins, A.T.A. ; Allinson, S.L. ; Short, R.D. / Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material. In: Journal of Physics D: Applied Physics. 2019 ; Vol. 52, No. 50.

Bibtex

@article{80db41a4eb8944958d0478a23c8fd4a9,
title = "Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material",
abstract = "The ability of plasma-generated hydrogen peroxide (H 2O 2) to traverse bacterial biofilms and the subsequent fate of the generated H 2O 2 has been investigated. An in vitro model, comprising a nanoporous membrane impregnated with artificial wound fluid and biofilms of varying maturity was treated with a helium-driven, cold atmospheric pressure plasma (CAP) jet. The concentration of H 2O 2 generated below the biofilms was quantified. The results showed that the plasma-generated H 2O 2 interacted significantly with the biofilm, thus exhibiting a reduction in concentration across the underlying nanoporous membrane. Biofilm maturity exhibited a significant effect on the penetration depth of H 2O 2, suggesting that well established, multilayer biofilms are likely to offer a shielding effect with respect to cells located in the lower layers of the biofilm, thus rendering them less susceptible to plasma disinfection. This may prove clinically significant in the plasma treatment of chronic, deep tissue infections such as diabetic and venous leg ulcers. Our results are discussed in the context of plasma-biofilm interactions, with respect to the fate of the longer lived reactive species generated by CAP, such as H 2O 2",
keywords = "biofilms, cold plasma, plasma, reactive species, Atmospheric pressure, Biological materials, Hydrogen peroxide, Oxidation, Peroxides, Plasma applications, Plasmas, Atmospheric pressure plasmas, Bacterial biofilm, Cold plasmas, Nanoporous membrane, Plasma treatment, Reactive species, Shielding effect, Venous leg ulcers, Biofilms",
author = "H.J. Hathaway and B.L. Patenall and N.T. Thet and A.C. Sedgwick and G.T. Williams and A.T.A. Jenkins and S.L. Allinson and R.D. Short",
note = "This is an author-created, un-copyedited version of an article accepted for publication/published in Journal of Physics D: Applied Physics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1361-6463/ab4539",
year = "2019",
month = "10",
day = "9",
doi = "10.1088/1361-6463/ab4539",
language = "English",
volume = "52",
journal = "Journal of Physics D: Applied Physics",
issn = "0022-3727",
publisher = "IOP Publishing Ltd",
number = "50",

}

RIS

TY - JOUR

T1 - Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material

AU - Hathaway, H.J.

AU - Patenall, B.L.

AU - Thet, N.T.

AU - Sedgwick, A.C.

AU - Williams, G.T.

AU - Jenkins, A.T.A.

AU - Allinson, S.L.

AU - Short, R.D.

N1 - This is an author-created, un-copyedited version of an article accepted for publication/published in Journal of Physics D: Applied Physics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi: 10.1088/1361-6463/ab4539

PY - 2019/10/9

Y1 - 2019/10/9

N2 - The ability of plasma-generated hydrogen peroxide (H 2O 2) to traverse bacterial biofilms and the subsequent fate of the generated H 2O 2 has been investigated. An in vitro model, comprising a nanoporous membrane impregnated with artificial wound fluid and biofilms of varying maturity was treated with a helium-driven, cold atmospheric pressure plasma (CAP) jet. The concentration of H 2O 2 generated below the biofilms was quantified. The results showed that the plasma-generated H 2O 2 interacted significantly with the biofilm, thus exhibiting a reduction in concentration across the underlying nanoporous membrane. Biofilm maturity exhibited a significant effect on the penetration depth of H 2O 2, suggesting that well established, multilayer biofilms are likely to offer a shielding effect with respect to cells located in the lower layers of the biofilm, thus rendering them less susceptible to plasma disinfection. This may prove clinically significant in the plasma treatment of chronic, deep tissue infections such as diabetic and venous leg ulcers. Our results are discussed in the context of plasma-biofilm interactions, with respect to the fate of the longer lived reactive species generated by CAP, such as H 2O 2

AB - The ability of plasma-generated hydrogen peroxide (H 2O 2) to traverse bacterial biofilms and the subsequent fate of the generated H 2O 2 has been investigated. An in vitro model, comprising a nanoporous membrane impregnated with artificial wound fluid and biofilms of varying maturity was treated with a helium-driven, cold atmospheric pressure plasma (CAP) jet. The concentration of H 2O 2 generated below the biofilms was quantified. The results showed that the plasma-generated H 2O 2 interacted significantly with the biofilm, thus exhibiting a reduction in concentration across the underlying nanoporous membrane. Biofilm maturity exhibited a significant effect on the penetration depth of H 2O 2, suggesting that well established, multilayer biofilms are likely to offer a shielding effect with respect to cells located in the lower layers of the biofilm, thus rendering them less susceptible to plasma disinfection. This may prove clinically significant in the plasma treatment of chronic, deep tissue infections such as diabetic and venous leg ulcers. Our results are discussed in the context of plasma-biofilm interactions, with respect to the fate of the longer lived reactive species generated by CAP, such as H 2O 2

KW - biofilms

KW - cold plasma

KW - plasma

KW - reactive species

KW - Atmospheric pressure

KW - Biological materials

KW - Hydrogen peroxide

KW - Oxidation

KW - Peroxides

KW - Plasma applications

KW - Plasmas

KW - Atmospheric pressure plasmas

KW - Bacterial biofilm

KW - Cold plasmas

KW - Nanoporous membrane

KW - Plasma treatment

KW - Reactive species

KW - Shielding effect

KW - Venous leg ulcers

KW - Biofilms

U2 - 10.1088/1361-6463/ab4539

DO - 10.1088/1361-6463/ab4539

M3 - Journal article

VL - 52

JO - Journal of Physics D: Applied Physics

JF - Journal of Physics D: Applied Physics

SN - 0022-3727

IS - 50

M1 - 505203

ER -