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    Rights statement: © The Author(s) 2013. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts

Research output: Contribution to journalJournal article

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Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts. / Taylor, Elaine; Bonsu-Dartnall, Nicola; Price, Jordan; Lindsay, Howard.

In: Nucleic Acids Research, Vol. 41, No. 16, 2013, p. 7725-7737.

Research output: Contribution to journalJournal article

Harvard

Taylor, E, Bonsu-Dartnall, N, Price, J & Lindsay, H 2013, 'Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts', Nucleic Acids Research, vol. 41, no. 16, pp. 7725-7737. https://doi.org/10.1093/nar/gkt549

APA

Vancouver

Author

Taylor, Elaine ; Bonsu-Dartnall, Nicola ; Price, Jordan ; Lindsay, Howard. / Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 16. pp. 7725-7737.

Bibtex

@article{aadc947a2a1045289db872c8ec82ff9c,
title = "Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts",
abstract = "UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) has a well-established role in epigenetic regulation through the recognition of various histone marks and interaction with chromatin-modifying proteins. However, its function in regulating cell cycle progression remains poorly understood and has been largely attributed to a role in transcriptional regulation. In this study we have used Xenopus laevis egg extracts to analyse Uhrf1 function in DNA replication in the absence of transcriptional influences. We demonstrate that removal of Uhrf1 inhibits chromosomal replication in this system. We further show that this requirement for Uhrf1, or an associated factor, occurs at an early stage of DNA replication and that the consequences of Uhrf1 depletion are not solely due to its role in loading Dnmt1 onto newly replicated DNA. We describe the pattern of Uhrf1 chromatin association before the initiation of DNA replication and show that this reflects functional requirements both before and after origin licensing. Our data demonstrate that the removal of Xenopus Uhrf1 influences the chromatin association of key replication proteins and reveal Uhrf1 as an important new factor required for metazoan DNA replication. ",
author = "Elaine Taylor and Nicola Bonsu-Dartnall and Jordan Price and Howard Lindsay",
note = "{\textcopyright} The Author(s) 2013. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.",
year = "2013",
doi = "10.1093/nar/gkt549",
language = "English",
volume = "41",
pages = "7725--7737",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "16",

}

RIS

TY - JOUR

T1 - Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts

AU - Taylor, Elaine

AU - Bonsu-Dartnall, Nicola

AU - Price, Jordan

AU - Lindsay, Howard

N1 - © The Author(s) 2013. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2013

Y1 - 2013

N2 - UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) has a well-established role in epigenetic regulation through the recognition of various histone marks and interaction with chromatin-modifying proteins. However, its function in regulating cell cycle progression remains poorly understood and has been largely attributed to a role in transcriptional regulation. In this study we have used Xenopus laevis egg extracts to analyse Uhrf1 function in DNA replication in the absence of transcriptional influences. We demonstrate that removal of Uhrf1 inhibits chromosomal replication in this system. We further show that this requirement for Uhrf1, or an associated factor, occurs at an early stage of DNA replication and that the consequences of Uhrf1 depletion are not solely due to its role in loading Dnmt1 onto newly replicated DNA. We describe the pattern of Uhrf1 chromatin association before the initiation of DNA replication and show that this reflects functional requirements both before and after origin licensing. Our data demonstrate that the removal of Xenopus Uhrf1 influences the chromatin association of key replication proteins and reveal Uhrf1 as an important new factor required for metazoan DNA replication.

AB - UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) has a well-established role in epigenetic regulation through the recognition of various histone marks and interaction with chromatin-modifying proteins. However, its function in regulating cell cycle progression remains poorly understood and has been largely attributed to a role in transcriptional regulation. In this study we have used Xenopus laevis egg extracts to analyse Uhrf1 function in DNA replication in the absence of transcriptional influences. We demonstrate that removal of Uhrf1 inhibits chromosomal replication in this system. We further show that this requirement for Uhrf1, or an associated factor, occurs at an early stage of DNA replication and that the consequences of Uhrf1 depletion are not solely due to its role in loading Dnmt1 onto newly replicated DNA. We describe the pattern of Uhrf1 chromatin association before the initiation of DNA replication and show that this reflects functional requirements both before and after origin licensing. Our data demonstrate that the removal of Xenopus Uhrf1 influences the chromatin association of key replication proteins and reveal Uhrf1 as an important new factor required for metazoan DNA replication.

U2 - 10.1093/nar/gkt549

DO - 10.1093/nar/gkt549

M3 - Journal article

VL - 41

SP - 7725

EP - 7737

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 16

ER -