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Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney. / Graham, Robert Iain; Rao, Shujing; Possee, Robert et al.
In: Journal of Invertebrate Pathology, Vol. 91, No. 2, 2006, p. 79-87.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Graham, RI, Rao, S, Possee, R, Sait, S, Mertens, P & Hails, R 2006, 'Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney', Journal of Invertebrate Pathology, vol. 91, no. 2, pp. 79-87. https://doi.org/10.1016/j.jip.2005.11.003

APA

Graham, R. I., Rao, S., Possee, R., Sait, S., Mertens, P., & Hails, R. (2006). Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney. Journal of Invertebrate Pathology, 91(2), 79-87. https://doi.org/10.1016/j.jip.2005.11.003

Vancouver

Graham RI, Rao S, Possee R, Sait S, Mertens P, Hails R. Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney. Journal of Invertebrate Pathology. 2006;91(2):79-87. doi: 10.1016/j.jip.2005.11.003

Author

Graham, Robert Iain ; Rao, Shujing ; Possee, Robert et al. / Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney. In: Journal of Invertebrate Pathology. 2006 ; Vol. 91, No. 2. pp. 79-87.

Bibtex

@article{73fd15c5fd9b4b7f8d7d835eb614f7d6,
title = "Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney",
abstract = "Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles in order to investigate the prevalence of virus pathogens. Reoviruses were isolated in 11 of the 13 winter moth populations sampled, with 3 novel species being detected. Two species of Cypoviridae (CPV) were isolated, Operophtera brumata CPV18 and O. brumata CPV19, with one host population suffering 46% infection prevalence of OpbuCPV19. A third virus, O. brumata Reovirus (OpbuRV), was isolated from both winter moth and a hymenopteran parasitoid wasp, Phobocampe tempestiva, which is abundant in these populations. This was identified as a non-occluded reovirus, which was clearly able to infect and persist in both the lepidopteran and the hymenopteran host. The genomes of the three viruses were characterised using gel electrophoresis and the virus structure was investigated using transmission electron microscopy. The relationship of these viruses with a baculovirus that also infects winter moth, OpbuNPV, was investigated, as well as the association of OpbuRV with P. tempestiva. The detection of such viruses is discussed with reference to studies of similar viruses in other lepidopteran and hymenopteran host systems.",
keywords = "Operophtera brumata, Cypovirus, Reovirus, Parasitoid, Phobocampe tempestiva, Nucleopolyhedrovirus",
author = "Graham, {Robert Iain} and Shujing Rao and Robert Possee and Steven Sait and Peter Mertens and Rosemary Hails",
year = "2006",
doi = "10.1016/j.jip.2005.11.003",
language = "English",
volume = "91",
pages = "79--87",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "ELSEVIER ACADEMIC PRESS INC",
number = "2",

}

RIS

TY - JOUR

T1 - Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata on Orkney

AU - Graham, Robert Iain

AU - Rao, Shujing

AU - Possee, Robert

AU - Sait, Steven

AU - Mertens, Peter

AU - Hails, Rosemary

PY - 2006

Y1 - 2006

N2 - Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles in order to investigate the prevalence of virus pathogens. Reoviruses were isolated in 11 of the 13 winter moth populations sampled, with 3 novel species being detected. Two species of Cypoviridae (CPV) were isolated, Operophtera brumata CPV18 and O. brumata CPV19, with one host population suffering 46% infection prevalence of OpbuCPV19. A third virus, O. brumata Reovirus (OpbuRV), was isolated from both winter moth and a hymenopteran parasitoid wasp, Phobocampe tempestiva, which is abundant in these populations. This was identified as a non-occluded reovirus, which was clearly able to infect and persist in both the lepidopteran and the hymenopteran host. The genomes of the three viruses were characterised using gel electrophoresis and the virus structure was investigated using transmission electron microscopy. The relationship of these viruses with a baculovirus that also infects winter moth, OpbuNPV, was investigated, as well as the association of OpbuRV with P. tempestiva. The detection of such viruses is discussed with reference to studies of similar viruses in other lepidopteran and hymenopteran host systems.

AB - Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles in order to investigate the prevalence of virus pathogens. Reoviruses were isolated in 11 of the 13 winter moth populations sampled, with 3 novel species being detected. Two species of Cypoviridae (CPV) were isolated, Operophtera brumata CPV18 and O. brumata CPV19, with one host population suffering 46% infection prevalence of OpbuCPV19. A third virus, O. brumata Reovirus (OpbuRV), was isolated from both winter moth and a hymenopteran parasitoid wasp, Phobocampe tempestiva, which is abundant in these populations. This was identified as a non-occluded reovirus, which was clearly able to infect and persist in both the lepidopteran and the hymenopteran host. The genomes of the three viruses were characterised using gel electrophoresis and the virus structure was investigated using transmission electron microscopy. The relationship of these viruses with a baculovirus that also infects winter moth, OpbuNPV, was investigated, as well as the association of OpbuRV with P. tempestiva. The detection of such viruses is discussed with reference to studies of similar viruses in other lepidopteran and hymenopteran host systems.

KW - Operophtera brumata

KW - Cypovirus

KW - Reovirus

KW - Parasitoid

KW - Phobocampe tempestiva

KW - Nucleopolyhedrovirus

U2 - 10.1016/j.jip.2005.11.003

DO - 10.1016/j.jip.2005.11.003

M3 - Journal article

VL - 91

SP - 79

EP - 87

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

IS - 2

ER -