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Determination of aspartate kinase in maize tissues.

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Determination of aspartate kinase in maize tissues. / Ferreira, Renato Rodrigues; Vendemiatti, Ariane; Gratão, Priscila Lupino et al.
In: Scientia Agricola, Vol. 62, No. 2, 2005, p. 184-189.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ferreira, RR, Vendemiatti, A, Gratão, PL, Lea, PJ & Azevedo, RA 2005, 'Determination of aspartate kinase in maize tissues.', Scientia Agricola, vol. 62, no. 2, pp. 184-189. https://doi.org/10.1590/S0103-90162005000200015

APA

Ferreira, R. R., Vendemiatti, A., Gratão, P. L., Lea, P. J., & Azevedo, R. A. (2005). Determination of aspartate kinase in maize tissues. Scientia Agricola, 62(2), 184-189. https://doi.org/10.1590/S0103-90162005000200015

Vancouver

Ferreira RR, Vendemiatti A, Gratão PL, Lea PJ, Azevedo RA. Determination of aspartate kinase in maize tissues. Scientia Agricola. 2005;62(2):184-189. doi: 10.1590/S0103-90162005000200015

Author

Ferreira, Renato Rodrigues ; Vendemiatti, Ariane ; Gratão, Priscila Lupino et al. / Determination of aspartate kinase in maize tissues. In: Scientia Agricola. 2005 ; Vol. 62, No. 2. pp. 184-189.

Bibtex

@article{de38cff8e54942c38246e6f84d5927f5,
title = "Determination of aspartate kinase in maize tissues.",
abstract = "Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.",
author = "Ferreira, {Renato Rodrigues} and Ariane Vendemiatti and Grat{\~a}o, {Priscila Lupino} and Lea, {Peter J.} and Azevedo, {Ricardo Antunes}",
year = "2005",
doi = "10.1590/S0103-90162005000200015",
language = "English",
volume = "62",
pages = "184--189",
journal = "Scientia Agricola",
issn = "1678-992X",
publisher = "Escola Superior de Agricultura Luiz de Queiroz",
number = "2",

}

RIS

TY - JOUR

T1 - Determination of aspartate kinase in maize tissues.

AU - Ferreira, Renato Rodrigues

AU - Vendemiatti, Ariane

AU - Gratão, Priscila Lupino

AU - Lea, Peter J.

AU - Azevedo, Ricardo Antunes

PY - 2005

Y1 - 2005

N2 - Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.

AB - Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.

U2 - 10.1590/S0103-90162005000200015

DO - 10.1590/S0103-90162005000200015

M3 - Journal article

VL - 62

SP - 184

EP - 189

JO - Scientia Agricola

JF - Scientia Agricola

SN - 1678-992X

IS - 2

ER -