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Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications

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Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications. / Liskova, Jana; Bacakova, Lucie; Skwarczynska, Agata L.; Musial, Olga; Bliznuk, Vitaliy; De Schamphelaere, Karel; Modrzejewska, Zofia; Douglas, Timothy E. L.

In: Journal of Functional Biomaterials, Vol. 6, No. 2, 06.2015, p. 192-203.

Research output: Contribution to journalJournal article

Harvard

Liskova, J, Bacakova, L, Skwarczynska, AL, Musial, O, Bliznuk, V, De Schamphelaere, K, Modrzejewska, Z & Douglas, TEL 2015, 'Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications', Journal of Functional Biomaterials, vol. 6, no. 2, pp. 192-203. https://doi.org/10.3390/jfb6020192

APA

Liskova, J., Bacakova, L., Skwarczynska, A. L., Musial, O., Bliznuk, V., De Schamphelaere, K., Modrzejewska, Z., & Douglas, T. E. L. (2015). Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications. Journal of Functional Biomaterials, 6(2), 192-203. https://doi.org/10.3390/jfb6020192

Vancouver

Liskova J, Bacakova L, Skwarczynska AL, Musial O, Bliznuk V, De Schamphelaere K et al. Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications. Journal of Functional Biomaterials. 2015 Jun;6(2):192-203. https://doi.org/10.3390/jfb6020192

Author

Liskova, Jana ; Bacakova, Lucie ; Skwarczynska, Agata L. ; Musial, Olga ; Bliznuk, Vitaliy ; De Schamphelaere, Karel ; Modrzejewska, Zofia ; Douglas, Timothy E. L. / Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications. In: Journal of Functional Biomaterials. 2015 ; Vol. 6, No. 2. pp. 192-203.

Bibtex

@article{9aa9bf685340443887c3e9ae2d98b5eb,
title = "Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications",
abstract = "Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit",
keywords = "chitosan, hydrogel, cytocompatibility, magnesium, mineralization",
author = "Jana Liskova and Lucie Bacakova and Skwarczynska, {Agata L.} and Olga Musial and Vitaliy Bliznuk and {De Schamphelaere}, Karel and Zofia Modrzejewska and Douglas, {Timothy E. L.}",
year = "2015",
month = jun
doi = "10.3390/jfb6020192",
language = "English",
volume = "6",
pages = "192--203",
journal = "Journal of Functional Biomaterials",
issn = "2079-4983",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

RIS

TY - JOUR

T1 - Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications

AU - Liskova, Jana

AU - Bacakova, Lucie

AU - Skwarczynska, Agata L.

AU - Musial, Olga

AU - Bliznuk, Vitaliy

AU - De Schamphelaere, Karel

AU - Modrzejewska, Zofia

AU - Douglas, Timothy E. L.

PY - 2015/6

Y1 - 2015/6

N2 - Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit

AB - Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit

KW - chitosan

KW - hydrogel

KW - cytocompatibility

KW - magnesium

KW - mineralization

U2 - 10.3390/jfb6020192

DO - 10.3390/jfb6020192

M3 - Journal article

VL - 6

SP - 192

EP - 203

JO - Journal of Functional Biomaterials

JF - Journal of Functional Biomaterials

SN - 2079-4983

IS - 2

ER -